cIAP (pan) Antibody (315301) - Pan Specific

Images

 
Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human cIAP‑1 or human cIAP‑2. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse cIAP Pan-specific Monoclonal ...read more
Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with either cIAP-1 or cIAP-2 and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western
Clone
315301
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

cIAP (pan) Antibody (315301) - Pan Specific Summary

Immunogen
E. coli-derived recombinant human cIAP-2
Asn2-Ser604
Accession # Q13489
Specificity
Detects human and mouse cIAP-1 and cIAP-2 in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 10 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
MAB3400 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for cIAP (pan) Antibody (315301) - Pan Specific

  • BIRC
  • cIAP
  • HIAP

Background

Cellular inhibitor of apoptosis protein1 (cIAP-1, also known as BIRC2, MIHB, and HIAP2) and 2 (cIAP-2, also known as BIRC3, MIHC, and HIAP1), are members of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. Structurally, cIAP-1 and cIAP-2 are each comprised of 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). Human cIAP-1 and cIAP-2 share 70% sequence identity. Functionally, cIAPs inhibits caspases through the direct interaction of its BIR domain with the active caspase. The ring finger domain of cIAP-1 and -2 also functions as an E3 ubiquitin ligase to ubiqutinylate specific target proteins. Caspase activity may be restored by mitochondrial proteins, such as SMAC/Diablo or HtrA2/Omi, through interactions with the Reaper-like motif and the BIR domain. cIAPs are reported to be cleaved by HtrA2/Omi.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for cIAP (pan) Antibody (MAB3400)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 2 applications: IHC-P, Western Blot.


Filter By Application
IHC-P
(1)
Western Blot
(9)
All Applications
Filter By Species
Human
(6)
Mouse
(3)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using MAB3400 Applications Species
T Goncharov, S Hedayati, MM Mulvihill, A Izrael-Tom, K Zobel, S Jeet, AV Fedorova, C Eidenschen, J deVoss, K Yu, AS Shaw, DS Kirkpatric, WJ Fairbrothe, K Deshayes, D Vucic Disruption of XIAP-RIP2 Association Blocks NOD2-Mediated Inflammatory Signaling Mol. Cell, 2018;69(4):551-565.e7. 2018 [PMID: 29452636] (Western Blot, Mouse) Western Blot Mouse
Characterization of Potent SMAC Mimetics that Sensitize Cancer Cells to TNF Family-Induced Apoptosis PLoS ONE, 2016;11(9):e0161952. 2016 [PMID: 27617834] (Western Blot, Human) Western Blot Human
N Rebolleda, I Losada-Fer, G Perez-Chac, R Castejon, S Rosado, M Morado, MT Vallejo-Cr, A Martinez, JA Vargas-Nuñ, P Perez-Acie Synergistic Activity of Deguelin and Fludarabine in Cells from Chronic Lymphocytic Leukemia Patients and in the New Zealand Black Murine Model PLoS ONE, 2016;11(4):e0154159. 2016 [PMID: 27101369] (Western Blot, Human) Western Blot Human
Moriwaki K, Bertin J, Gough P, Chan F A RIPK3-caspase 8 complex mediates atypical pro-IL-1beta processing. J Immunol, 2015;194(4):1938-44. 2015 [PMID: 25567679] (Western Blot, Mouse) Western Blot Mouse
Zhang L, Dittmer M, Blackwell K, Workman L, Hostager B, Habelhah H TRAIL activates JNK and NF-kappaB through RIP1-dependent and -independent pathways. Cell Signal, 2015;27(2):306-14. 2015 [PMID: 25446254] (Western Blot, Human) Western Blot Human
Faye M, Beug S, Graber T, Earl N, Xiang X, Wild B, Langlois S, Michaud J, Cowan K, Korneluk R, Holcik M IGF2BP1 controls cell death and drug resistance in rhabdomyosarcomas by regulating translation of cIAP1. Oncogene, 2015;34(12):1532-41. 2015 [PMID: 24704827] (IHC-P, Human) IHC-P Human
Moreno-Garcia M, Sommer K, Rincon-Arano H, Brault M, Ninomiya-Tsuji J, Matesic L, Rawlings D Kinase-independent feedback of the TAK1/TAB1 complex on BCL10 turnover and NF-kappaB activation. Mol Cell Biol, 2013;33(6):1149-63. 2013 [PMID: 23297344] (Western Blot, Chicken) Western Blot Chicken
Perez-Chacon G, Llobet D, Pardo C, Pindado J, Choi Y, Reed JC, Zapata JM TNFR-Associated Factor 2 Deficiency in B Lymphocytes Predisposes to Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma in Mice. J. Immunol., 2012;189(2):1053-61. 2012 [PMID: 22711886] (Western Blot, Mouse) Western Blot Mouse
Gerlach B, Cordier SM, Schmukle AC, Emmerich CH, Rieser E, Haas TL, Webb AI, Rickard JA, Anderton H, Wong WW, Nachbur U, Gangoda L, Warnken U, Purcell AW, Silke J, Walczak H Linear ubiquitination prevents inflammation and regulates immune signalling. Nature, 2011;471(7340):591-6. 2011 [PMID: 21455173] (Western Blot, Human) Western Blot Human
Jin HR, Jin X, Lee JJ Zinc-finger protein 91 plays a key role in LIGHT-induced activation of non-canonical NF-kappaB pathway. Biochem. Biophys. Res. Commun., 2010;400(4):581-6. 2010 [PMID: 20804734] (Western Blot, Human) Western Blot Human

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