cIAP (pan) Antibody (315301) [Alexa Fluor® 488]

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 315301
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

cIAP (pan) Antibody (315301) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human cIAP-2
  Asn2-Ser604
  Accession # Q13489
• Specificity: Detects human and mouse cIAP-1 and cIAP-2 in Western blots.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for cIAP (pan) Antibody (315301) [Alexa Fluor® 488]

• AIP1;API2;Baculoviral IAP repeat-containing protein 3;BIRC3;CIAP2;c-IAP2;HAIP1;HIAP1;IAP-1;MALT2;MIHC;RNF49
• BIRC
• cIAP
• HIAP

Background

Cellular inhibitor of apoptosis protein1 (cIAP-1, also known as BIRC2, MIHB, and HIAP2) and 2 (cIAP-2, also known as BIRC3, MIHC, and HIAP1), are members of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. Structurally, cIAP-1 and cIAP-2 are each comprised of 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). Human cIAP-1 and cIAP-2 share 70% sequence identity. Functionally, cIAPs inhibits caspases through the direct interaction of its BIR domain with the active caspase. The ring finger domain of cIAP-1 and -2 also functions as an E3 ubiquitin ligase to ubiqutinylate specific target proteins. Caspase activity may be restored by mitochondrial proteins, such as SMAC/Diablo or HtrA2/Omi, through interactions with the Reaper-like motif and the BIR domain. cIAPs are reported to be cleaved by HtrA2/Omi.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.