Chymase/CMA1/Mast Cell Chymase Antibody

Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody [NBP1-52386] - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary

Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP1-52386] - Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast tissue.

Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP1-52386] - Analysis of anti-Mast Cell Chymase antibody with human tonsil, mast cells at concentration 1.5 ug/ml.

Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP1-52386] - Analysis of anti-Mast Cell Chymase antibody with human testis, mast cells at concentration 1.5 ug/ml.

Product Discontinued

Chymase/CMA1/Mast Cell Chymase Antibody Summary

• Immunogen: Peptide with sequence C-QFRHPKYNTSTLHHD, from the internal region of the Chymase/CMA1/Mast Cell Chymase protein sequence according to NP_001827.1.
• Predicted Species: Primate (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CMA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA 1:128000
  • Western Blot 0.5 - 1 ug/ml

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gibbon (100%), Baboon (100%), Gorilla (93%), Marmoset (87%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: TBS (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Chymase/CMA1/Mast Cell Chymase Antibody

• alpha-chymase
• chymase 1 preproprotein transcript E
• chymase 1 preproprotein transcript I
• chymase 1, mast cell
• Chymase
• chymase, heart
• chymase, mast cell
• CMA1
• CYH
• CYM
• EC 3.4.21
• EC 3.4.21.39
• Mast cell protease I
• MCP3P
• Mcpt5
• MCT1
• MGC119890
• MGC119891

Background

Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CMA1
• Entrez:
  • 1215(Human)
• Uniprot:
  • P23946()