Chromogranin A Antibody - BSA Free

Immunohistochemistry-Paraffin: Chromogranin A Antibody [NB100-62464] - Paraffin embedded human pancreas with Sheep anti Human Chromogranin following pronase antigen retrieval.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 5 mg/ml

Chromogranin A Antibody - BSA Free Summary

• Immunogen: Synthetic peptide corresponding to the amino acids 1 - 15 (lpvnspmnkgdtevm) of the native molecule conjugated to Keyhole limpet Haemocyanin
• Epitope: L-P-V-N-S-P-M-N-K-G-D-T-E-V-M
• Localization: Neuroendocrine and endocrine secretory granules.
• Specificity: This product recognizes human chromogranin A, a secreted peptide that inhibits glucose-induced insulin release.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CHGA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:25-1:100
• Application Notes: For IHC, Pronase protein digestion pre-treatment of paraffin sections is required.
• Control:

  Chromogranin A Overexpression Lysate

Reactivity Notes

Predicted cross-reactivities: Bovine, Chimpanzee, Equine, Dog, Mouse, Porcine, Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 5 mg/ml
• Purity: Protein G purified

Alternate Names for Chromogranin A Antibody - BSA Free

• betagranin (N-terminal fragment of chromogranin A)
• Betagranin
• Catestatin
• CGA
• CHGA
• Chromofungin
• chromogranin A (parathyroid secretory protein 1)
• Chromogranin A
• chromogranin-A
• Pancreastatin
• Parastatin
• Parathyroid Secretory Protein 1
• Pituitary Secretory Protein I
• SP-I

Background

Chromogranin A (a protein of 439 amino acids which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Chromogranin A Antibody (NB100-62464). (Showing 1 - 1 of 1 FAQ).

1. I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?
   • When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.

Bioinformatics

• Gene Symbol: CHGA
• Entrez:
  • 1113(Human)
• Uniprot:
  • P10645()
  • P10645()