Chromogranin A Antibody (LK2H10)

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) [NBP2-32956] - Mouse Pancreas stained with Chromogranin A Antibody (LK2H10).

Flow Cytometry: Chromogranin A Antibody (LK2H10) [NBP2-32956] - Flow Cytometry: Chromogranin A Antibody (LK2H10) [Alexa Fluor® 647] [NBP2-34672AF647] - An intracellular stain was performed on SH-SY5Y cells with Chromogranin A Antibody (LK2H10) NBP2-34672AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 form of this antibody.

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) [NBP2-32956] - Formalin-fixed, paraffin-embedded human small cell lung carcinoma stained with Chromogranin A MAb (LK2H10)

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) [NBP2-32956] - Formalin-fixed, paraffin-embedded human Adrenal Gland stained with Chromogranin A Monoclonal Antibody (LK2H10)

• Reactivity: Hu, Mu, Rt, Po, Pm, Gp(-), Rb(-), Sh(-)
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: LK2H10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Chromogranin A Antibody (LK2H10) Summary

• Immunogen: Human pheochromocytoma
• Localization: Finely granular cytoplasmic
• Marker: Neuroendocrine Marker
• Specificity: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHGA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
• Application Notes: ELISA (For coating, order NBP2-34672 (BSA free version of NBP2-32956)). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for Chromogranin A Antibody (LK2H10)

• betagranin (N-terminal fragment of chromogranin A)
• Betagranin
• Catestatin
• CGA
• CHGA
• Chromofungin
• chromogranin A (parathyroid secretory protein 1)
• Chromogranin A
• chromogranin-A
• Pancreastatin
• Parastatin
• Parathyroid Secretory Protein 1
• Pituitary Secretory Protein I
• SP-I

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Chromogranin A Antibody (NBP2-32956)(1)

Publication using NBP2-32956

• Sayadi LR, Alexander M, Sorensen AM et al Micro/nanobubbles: Improving Pancreatic Islet Cell Survival for Transplantation. Ann Plast Surg. 2019 Nov [PMID: 31232817]
  
  Details:
  Citation using the Azide and BSA Free version of this antibody.

FAQs for Chromogranin A Antibody (NBP2-32956). (Showing 1 - 1 of 1 FAQ).

1. I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?
   • When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.

Bioinformatics

• Gene Symbol: CHGA
• Uniprot:
  • P10645()