Chordin-like 1/CHRDL1 Antibody - BSA Free

Western Blot: Chordin-like 1/CHRDL1 Antibody [NBP3-12874] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Chordin-like 1/CHRDL1 antibody (NBP3-12874) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Chordin-like 1/CHRDL1 Antibody [NBP3-12874] - Chordin-like 1/CHRDL1 antibody detects Chordin-like 1/CHRDL1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Chordin-like 1/CHRDL1 antibody (NBP3-12874) dilution: 1:500. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Immunohistochemistry-Paraffin: Chordin-like 1/CHRDL1 Antibody [NBP3-12874] - Chordin-like 1/CHRDL1 antibody detects Chordin-like 1/CHRDL1 protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. Chordin-like 1/CHRDL1 antibody (NBP3-12874) dilution: 1:500. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Western Blot: Chordin-like 1/CHRDL1 Antibody [NBP3-12874] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Chordin-like 1/CHRDL1 antibody (NBP3-12874) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: Chordin-like 1/CHRDL1 Antibody [NBP3-12874] - Chordin-like 1/CHRDL1 antibody detects Chordin-like 1/CHRDL1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Chordin-like 1/CHRDL1 stained by Chordin-like 1/CHRDL1 antibody (NBP3-12874) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10um.

• Reactivity: Hu, Mu, Rt, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Chordin-like 1/CHRDL1 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Chordin-like 1/CHRDL1. The exact sequence is proprietary.
• Predicted Species: Rat (93%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHRDL1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Chicken (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Chordin-like 1/CHRDL1 Antibody - BSA Free

• CHL
• Chordin-like 1
• chordin-like protein 1
• CHRDL1
• CRDL1
• dA141H5.1
• Neuralin 1
• neuralin-1
• neurogenesin-1
• NRLN1
• NRLN1neuralin 1
• Ventroptin alpha
• ventroptin
• VOPT

Background

CHRDL1 encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHRDL1