Choline Kinase beta Antibody

Images

 
Western Blot: Choline Kinase beta Antibody [H00001120-B01P] - Analysis of CHKB expression in transfected 293T cell line by CHKB polyclonal antibody. Lane 1: CHKB transfected lysate(43.45 KDa). Lane 2: Non-transfected ...read more
Immunocytochemistry/ Immunofluorescence: Choline Kinase beta Antibody [H00001120-B01P] - Analysis of purified antibody to CHKB on HeLa cell. (antibody concentration 10 ug/ml)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Choline Kinase beta Antibody Summary

Immunogen
CHKB (NP_005189.2, 1 a.a. - 395 a.a.) full-length human protein. MAAEATAVAGSGAVGGCLAKDGLQQSKCPDTTPKRRRASSLSRDAERRAYQWCREYLGGAWRRVQPEELRVYPVSGGLSNLLFRCSLPDHLPSVGEEPREVLLRLYGAILQGVDSLVLESVMFAILAERSLGPQLYGVFPEGRLEQYIPSRPLKTQELREPVLSAAIATKMAQFHGMEMPFTKEPHWLFGTMERYLKQIQDLPPTGLPEMNLLEMYSLKDEMGNLRKLLESTPSPVVFCHNDIQEGNILLLSEPENADSLMLVDFEYSSYNYRGFDIGNHFCEWVYDYTHEEWPFYKARPTDYPTQEQQLHFIRHYLAEAKKGETLSQEEQRKLEEDLLVEVSRYALASHFFWGLWSILQASMSTIEFGYLDYAQSRFQFYFQQKGQLTSVHSSS
Specificity
Reacts with choline kinase beta.
Clonality
Polyclonal
Host
Mouse
Gene
CHKB
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This antibody is reactive against transfected lysate in WB, IF and as a detection antibody in ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Choline Kinase beta Antibody

  • CHETK
  • CHETKcholine/ethanolamine kinase beta
  • CHKL
  • CHKLcholine kinase-like
  • ChoKB
  • Choline Kinase beta
  • choline kinase betaEK
  • Choline kinase-like protein
  • CK
  • CKB
  • CKEKB
  • CKEKBEKBcholine/ethanolamine kinase
  • EC 2.7.1.32
  • EC 2.7.1.82
  • EK
  • EKB
  • Ethanolamine Kinase beta
  • Ethanolamine kinase
  • MDCMC

Background

Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CHKB
Entrez
Uniprot

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