Choline Kinase beta Antibody - Azide and BSA Free

Western Blot: Choline Kinase beta Antibody [H00001120-B01P] - Analysis of CHKB expression in transfected 293T cell line by CHKB polyclonal antibody. Lane 1: CHKB transfected lysate(43.45 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Choline Kinase beta Antibody [H00001120-B01P] - Analysis of purified antibody to CHKB on HeLa cell. (antibody concentration 10 ug/ml)

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Choline Kinase beta Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: CHKB (NP_005189.2, 1 a.a. - 395 a.a.) full-length human protein. MAAEATAVAGSGAVGGCLAKDGLQQSKCPDTTPKRRRASSLSRDAERRAYQWCREYLGGAWRRVQPEELRVYPVSGGLSNLLFRCSLPDHLPSVGEEPREVLLRLYGAILQGVDSLVLESVMFAILAERSLGPQLYGVFPEGRLEQYIPSRPLKTQELREPVLSAAIATKMAQFHGMEMPFTKEPHWLFGTMERYLKQIQDLPPTGLPEMNLLEMYSLKDEMGNLRKLLESTPSPVVFCHNDIQEGNILLLSEPENADSLMLVDFEYSSYNYRGFDIGNHFCEWVYDYTHEEWPFYKARPTDYPTQEQQLHFIRHYLAEAKKGETLSQEEQRKLEEDLLVEVSRYALASHFFWGLWSILQASMSTIEFGYLDYAQSRFQFYFQQKGQLTSVHSSS
• Specificity: Reacts with choline kinase beta.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: CHKB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: This antibody is reactive against transfected lysate in WB, IF and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Choline Kinase beta Antibody - Azide and BSA Free

• CHETK
• CHETKcholine/ethanolamine kinase beta
• CHKL
• CHKLcholine kinase-like
• ChoKB
• Choline Kinase beta
• choline kinase betaEK
• Choline kinase-like protein
• CK
• CKB
• CKEKB
• CKEKBEKBcholine/ethanolamine kinase
• EC 2.7.1.32
• EC 2.7.1.82
• EK
• EKB
• Ethanolamine Kinase beta
• Ethanolamine kinase
• MDCMC

Background

Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHKB
• Entrez:
  • 1120(Human)
• Uniprot:
  • NP_005189.2(Human)