Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free

Analysis in human placenta and liver tissues using NBP3-43796 antibody. Corresponding Choline Acetyltransferase/ChAT RNA-seq data are presented for the same tissues.

Staining of human cerebral cortex shows moderate positivity in cholinergic neural fibers.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human placenta shows strong cytoplasmic positivity in a subset of cells in chorionic villi.

Staining of rat brain shows strong positivity in acetylcholine neurons in the basal forebrain.

Staining of mouse brain shows strong positivity in acetylcholine neurons in the basal forebrain.

Staining of mouse basal forebrain shows strong positivity in acetylcholine neurons in the caudate putamen.

Staining of rat brain shows strong positivity in acetylcholine neurons in the medial septum.

Staining of SK-MEL-30 cells using the Anti-Choline Acetyltransferase/ChAT monoclonal antibody) .

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clone: CL3173
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free (NBP2-46620) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P28329, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHAT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free

• acetyl CoA:choline O-acetyltransferase
• CHAT
• CHOACTase
• Choline acetylase
• Choline Acetyltransferase
• choline O-acetyltransferase
• CMS1A
• CMS1A2
• EC 2.3.1
• EC 2.3.1.6

Background

Note: This is the same product as NB300-178. FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHAT