Chk1 Antibody - BSA Free

Western Blot: Chk1 Antibody [NB100-274] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: Chk1 Antibody [NB100-274] - Whole cell lysate (30 ug) from HeLa cells treated with 10 Gy ionizing radiation (IR) or untreated. Antibody used at 0.1 ug/ml.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Chk1 Antibody - BSA Free Summary

• Description: Novus Biologicals Goat Chk1 Antibody - BSA Free (NB100-274) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residues 200 and 250 of human Checkpoint Kinase 1 using the numbering given in entry NP_001265.1 (GeneID 1111).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CHEK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot 1:2500-1:15000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Chk1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Chk1 Antibody - BSA Free

• Checkpoint, S. pombe, homolog of, 1
• CHEK1
• CHK1 (checkpoint, S.pombe) homolog
• CHK1 checkpoint homolog (S. pombe)
• Chk1
• CHK1serine/threonine-protein kinase Chk1
• EC 2.7.11
• EC 2.7.11.1

Background

In response to DNA damage and replication blocks, cells prevent cell cycle progression through the control of critical cell cycle regulators. Chk1 is an essential kinase for embryonic development and regulating mitotic progression in response to DNA damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHEK1
• Entrez:
  • 1111(Human)
• Uniprot:
  • O14757()