Chk1 Antibody

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Western blot shows lysates of NRK rat normal kidney cell line, U2OS human osteosarcoma cell line, MCF‑7 human breast cancer cell line, CEM human T‑lymphoblastoid cell line, and Balb/3T3 mouse embryonic fibroblast ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Chk1 Antibody Summary

Immunogen
E. coli-derived recombinant human Chk1
Tyr157-Thr476
Accession # O14757
Specificity
Detects human, mouse, and rat Chk1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CHEK1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunoprecipitation 2 ug/500 ug cell lysate
Publications
Read Publication using
AF1630 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chk1 Antibody

  • Checkpoint, S. pombe, homolog of, 1
  • CHEK1
  • CHK1 (checkpoint, S.pombe) homolog
  • CHK1 checkpoint homolog (S. pombe)
  • Chk1
  • CHK1serine/threonine-protein kinase Chk1
  • EC 2.7.11
  • EC 2.7.11.1

Background

The Chk1 checkpoint kinase is an integral member of a signaling cascade that controls cell cycle progression. In response to genotoxic stress, Chk1 is phosphorylated by ATM or ATM-related kinases. In turn, Chk1 phosphorylates downstream effectors, such as p53 or the Cdc25 phosphatases to halt cell cycle progression and allow time for repair of incurred damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Chk1 Antibody (AF1630)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CHEK1
Uniprot