Chk1 [p Ser296] Antibody (SN06-50)

Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human tonsil tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human breast carcinoma tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: SN06-50
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Chk1 [p Ser296] Antibody (SN06-50) Summary

• Description: Novus Biologicals Rabbit Chk1 [p Ser296] Antibody (SN06-50) (NBP2-67711) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Chk1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser296 of human Chk1 (SwissProt: O14757 Human)
• Modification: p Ser296
• Localization: Chromosome, Cytoplasm, Cytoskeleton, Nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CHEK1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Chk1 [p Ser296] Antibody (SN06-50)

• Checkpoint, S. pombe, homolog of, 1
• CHEK1
• CHK1 (checkpoint, S.pombe) homolog
• CHK1 checkpoint homolog (S. pombe)
• Chk1
• CHK1serine/threonine-protein kinase Chk1
• EC 2.7.11
• EC 2.7.11.1

Background

Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Chk1 Antibody (NBP2-67711)(1)

Publication using NBP2-67711

• Fu K, Sun X, Xia X et al. Sam68 is required for the growth and survival of nonmelanoma skin cancer Cancer Med 2019-10-01 [PMID: 31436046] (WB, Human)

Review for Chk1 Antibody (NBP2-67711) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 08/05/2020

Summary

• Application: Western Blot
• Sample Tested: Human Pancreatic Cancer Cell line
• Species: Human
• Lot: HJ0629

Comments

• Comments: L3.6 PDAC cell line was treated 48 hours with DMSO or the Chk1 inhibitor LY2606368.
  Chk1 p Ser296 primary antibody dilution 1:1000
  Anti rabbit HRP conjugated secondary dilution 1:2000
  Blocking performed using 5% BSA on TBS-Tween solution

Bioinformatics

• Gene Symbol: CHEK1