Chitotriosidase/CHIT1 Antibody - BSA Free

Chitotriosidase-CHIT1-Antibody-Immunohistochemistry-Paraffin-NBP1-84490-img0010.jpg

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.

Staining of human lung shows strong cytoplasmic positivity in granulocytes.

Analysis in human lung and liver tissues using HPA010575 antibody. Corresponding CHIT1 RNA-seq data are presented for the same tissues.

Analysis in human lung tissue.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Chitotriosidase/CHIT1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: FDDVESFKTKVSYLKQKGLGGAMVWALDLDDFAGFSCNQGRYPLIQTLRQELSLPYLPSGTPELEVPKPGQPSEPEHGPSPGQDTFCQGKADGLYPNPRERSSFYSCAAGRLFQQSCPTGLVFSNSCKCCTWN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHIT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Chitotriosidase/CHIT1 Protein (NBP1-84490PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Chitotriosidase/CHIT1 Antibody - BSA Free

• CHI3
• CHIT1
• CHITCHI3
• chitinase 1 (chitotriosidase)
• chitinase-1
• Chitotriosidase
• chitotriosidase-1
• EC 3.2.1.14
• FLJ00314
• MGC125322
• plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase

Background

Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Chitotriosidase/CHIT1 Antibody (NBP1-84490)(1)

Publication using NBP1-84490

• Hendrickx DAE, van Scheppingen J, van der Poel M et al. Gene Expression Profiling of Multiple Sclerosis Pathology Identifies Early Patterns of Demyelination Surrounding Chronic Active Lesions Front Immunol 2017-12-21 [PMID: 29312322] (IHC-P, Human)

Bioinformatics

• Gene Symbol: CHIT1