Chitinase 3-like 1/YKL-40 Antibody (321924) [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: WB
• Clone: 321924
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 594

Chitinase 3-like 1/YKL-40 Antibody (321924) [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Chitinase 3-like 1/YKL-40
  Tyr22-Ala381
  Accession # Q61362
• Specificity: Detects mouse Chitinase 3-like 1/YKL-40 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human Chitinase 3-like 1/YKL-40.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chitinase 3-like 1/YKL-40 Antibody (321924) [Alexa Fluor® 594]

• AW208766
• Brp39
• CHI3L1
• Chitinase 3 like 1
• Chitinase 3-like 1
• gp39
• HCgp39
• YKL-40

Background

Chitinase 3-like 1 (CHI3L1), also called breast regression protein 39 (BRP39) in mouse, or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1-4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5-7). The mouse CHI3L1 cDNA encodes 381 amino acids (aa), including a 21 aa signal sequence and a 360 aa mature region with two intermolecular disulfides (3). Mature mouse CHI3L1 shares 73%, 75%, 72%, 71%, 70% and 69% aa sequence identity with human, rat, equine, porcine, canine and bovine CHI3L1, respectively. CHI3L1 does not show chitotriosidase activity, but binds chitin and is thus termed a chi-lectin (1-4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6-9). Human elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9-12). Human single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15). Mice lacking CHI3L1 have markedly diminished antigen-induced Th2 responses, which can be rescued by expression of human CHI3L1 (16).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.