Cerebellin-1/Precerebellin Antibody

Images

 
Western Blot: Cerebellin-1/Precerebellin Antibody [NBP1-77238] - Western blot analysis of precerebellin in 293 cell lysate with precerebellin antibody at (A) 2 and (B) 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: Cerebellin-1/Precerebellin Antibody [NBP1-77238] - 3T3 cells with precerebellin antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Cerebellin-1/Precerebellin Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy- terminus human precerebellin. The immunogen is located within the last 50 amino acids of Precerebellin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CBLN1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
Precerebellin antibody can be used for the detection of precerebellin by Western blot at 2 - 4 mg/ml.
Control Peptide
Cerebellin-1/Precerebellin Peptide (NBP1-77238PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-77238 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cerebellin-1/Precerebellin Antibody

  • CBLN1
  • cerebellin 1 precursor
  • Cerebellin1
  • Cerebellin-1
  • CLN1
  • Precerebellin

Background

Precerebellin is the precursor of the brain-specific hexadecapeptide cerebellin, a protein with substantial similarity to the globular region of the B chain of complement component C1q. Cerebellin exerts neuromodulatory functions by directly stimulating norepinephrine release via the adenylate cyclase/pka- dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release. The active form of precerebellin is highly enriched in postsynaptic structures of cerebellar Purkinje cells in cartwheel neurons of the dorsal cochlear nucleus. Because precerebellin belongs to the C1q/tumor necrosis factor superfamily of secreted proteins and has similarity to adiponectin and CTRP3, it has been suggested that precerebellin posseses functions other than those already stated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Cerebellin-1/Precerebellin Antibody (NBP1-77238) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-77238:
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Images Ratings Applications Species Date Details
Western Blot Cerebellin-1/Precerebellin NBP1-77238
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reviewed by:
David Bodznick
WB Other 02/09/2016
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Summary

ApplicationWestern Blot
Sample TestedSkate brain
SpeciesOther

Blocking

Blocking Details1 Hour at RT in NGS

Secondary Antibody

Secondary Descriptionanti-rb

Details

Detection NotesHRP secondary with FEMTO. 28 second exposure time

Comments

CommentsWorked great with minimal complications in a basal vertebrate.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cerebellin-1/Precerebellin Antibody (NBP1-77238) (0)

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Secondary Antibodies

 

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

David Bodznick
02/09/2016
Application: WB
Species: Other

Bioinformatics

Gene Symbol CBLN1
Entrez
Uniprot