Recombinant Human Cerebellin-2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Cerebellin-2 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Cerebellin-2 is coated at 5 μg/mL, biotinylated recombinant rat NRXN-1 beta binds with an apparent K_D <1.5 nM. Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Cerebellin-2, immobilized at 3-30 μg/mL, is able to significantly enhance neurite outgrowth.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Cerebellin-2 protein
  Arg50-Leu224, with an N-terminal HA (YPYDVPDYA) tag
• Accession #: Q8IUK8
• N-terminal Sequence: Tyr
• Protein/Peptide Type: Recombinant Proteins
• Gene: CBLN2
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 25-35 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Cerebellin-2 Protein, CF

• CBLN2
• cerebellin 2 precursor
• Cerebellin2
• Cerebellin-2

Background

Cerebellin-2 (CBLN2) is an approximately 25 kDa N-glycosylated protein in the Cerebellin family of TNF superfamily molecules (1). Cerebellins contain a collagenous domain and a C-terminal TNF/C1q-like domain. Human Cerebellin-2 consists of this 175 amino acid (aa) region following an N-terminal 28 aa potential cytoplasmic domain and a 21 aa potential transmembrane segment. Within the Cerebellin-like region, human Cerebellin-2 shares 99% aa sequence identity with mouse, rat, and chick Cerebellin-2 (2‑4). It is expressed in multiple regions of the nervous system including the neocortex, olfactory bulb, thalamus, hypothalamus, midbrain (5), preganglionic sensory nerves, dorsal root ganglia, and sensory and motoneurons of the spinal cord (4, 6) as well as in the zona glomerulosa of the adrenal cortex (7). Cerebellin-2 forms cell associated homo-oligomers and heteromers with Cerebellins-1, -3, and -4 [Iijima 1049]. It binds with high affinity to alpha and beta Neurexin 1 isoforms that contain the splice site 4 insert (+SS4) and more weakly to Neurexin 2 beta (+SS4) and Neurexin 3 beta (+SS4) (9, 10). These interactions promote presynaptic differentiation in cultures of cortical and cerebellar neurons (9, 10).

1. Yuzaki, M. (2011) Curr. Opin. Neurobiol. 21:215.
2. Kavety, B. et al. (1994) Brain Res. Mol. Brain Res. 27:152.
3. Wada, C. and H. Ohtani (1991) Brain Res. Mol. Brain Res. 9:71.
4. Yang, M. et al. (2010) J. Comp. Neurol. 518:2818.
5. Miura, E. et al. (2006) Eur. J. Neurosci. 24:750.
6. Reiner, A. et al. (2011) J. Comp. Neurol. 519:2225.
7. Rucinski, M. et al. (2005) Int. J. Mol. Med. 15:411.
8. Iijima, T. et al. (2007) Eur. J. Neurosci. 25:1049.
9. Joo, J.-Y. et al. (2011) Biochem. Biophys. Res. Commun. 406:627.
10. Matsuda, K. and M. Yuzaki (2011) Eur. J. Neurosci. 33:1447.

Publications for Cerebellin-2 (7044-CB)(2)

Publications using 7044-CB

• PJ Gandhi, DY Gawande, GP Shelkar, SG Gakare, T Kiritoshi, G Ji, B Misra, R Pavuluri, J Liu, V Neugebauer, SM Dravid Dysfunction of Glutamate Delta-1 Receptor-Cerebellin 1 Trans-Synaptic Signaling in the Central Amygdala in Chronic Pain Cells, 2021-10-03;10(10):. 2021-10-03 [PMID: 34685624] (In Vivo, Mouse)
• Haddick P, Tom I, Luis E, Quinones G, Wranik B, Ramani S, Stephan J, Tessier-Lavigne M, Gonzalez L Defining the ligand specificity of the deleted in colorectal cancer (DCC) receptor. PLoS ONE, 2014-01-06;9(1):e84823. 2014-01-06 [PMID: 24400119] (Surface Plasmon Resonance)

Bioinformatics

• Gene Symbol: CBLN2
• Uniprot:
  • Q8IUK8()