CEP72 Antibody

Western Blot: CEP72 Antibody [NB100-60661] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: NB100-60661 used for IP at 6 ug per reaction (Lane 3). CEP72 was also immunoprecipitated by a previous lot of this antibody and NB100-60662 (Lane 2). For blotting immunoprecipitated CEP72, NB100-60661 was used at 0.04 ug/ml (Lane 1). Detection: Chemiluminescence with an exposure time of 75 seconds.

Western Blot: CEP72 Antibody [NB100-60661] - Whole cell lysate (15 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CEP72 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB, IHC, IHC-Fr, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CEP72 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 150 and 200 of centrosomal protein 72kDa using the numbering given in entry NP_060610.2 (GeneID 55722).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CEP72
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 20228799).
• Control:

  CEP72 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CEP72 Antibody

• centrosomal protein 72kDa
• centrosomal protein of 72 kDa
• Cep72
• KIAA1519FLJ10565
• MGC5307

Background

CEP72 is a centrosomal protein that belongs to the leucine-rich-repeat (LRR) superfamily of proteins. The specific function of CEP72 has not been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEP72 Antibody (NB100-60661)(2)

Publications using NB100-60661

• Stowe TR, Wilkinson CJ, Iqbal A et al. The centriolar satellite proteins Cep72 and Cep290 interact and are required for recruitment of BBS proteins to the cilium. Mol Biol Cell 2012-09-01 [PMID: 22767577]
• McGovern DP, Gardet A, Torkvist. Genome-wide association identifies multiple ulcerative colitis susceptibility loci. Nat Genet. 2010-04-01 [PMID: 20228799] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: CEP72
• Entrez:
  • 55722(Human)
• Uniprot:
  • NP_060610.2(Human)