SEC6 Antibody (9H5)

Images

 
Western Blot: SEC6 Antibody (9H5) [NBP1-97500] - Establishment and validation of tagged exocyst subunit cell lines. Western blots with subunit specific antibodies show successful incorporation of sfGFP in both alleles ...read more
Immunohistochemistry-Paraffin: SEC6 Antibody (9H5) [NBP1-97500] - Analysis of human tonsil tissue stained with rSec6, mAb (9H5) at 10ug/ml.
Western Blot: SEC6 Antibody (9H5) [NBP1-97500] - Analysis of pig ESK-4 cell lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Ha, Pm, Rb, Sh, YeSpecies Glossary
Applications WB, IB, ICC/IF, IHC, IHC-P
Clone
9H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SEC6 Antibody (9H5) Summary

Immunogen
Recombinant rat Sec6.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
EXOC3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Detects a band of ~87kDa by Western blot. Use in Immunoblotting reported in scientific literature (PMID 28358000).
Publications
Read Publications using
NBP1-97500 in the following applications:

  • IB
    2 publications
  • 1 publication
  • WB
    3 publications

Reactivity Notes

Yeast reactivity reported in scientific literature (PMID: 30510181).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Alternate Names for SEC6 Antibody (9H5)

  • exocyst complex component 3
  • Exocyst complex component Sec6
  • Sec 6 homolog
  • SEC6
  • SEC6L1
  • SEC6-like 1 (S. cerevisiae)
  • SEC6-like 1
  • Sec6p

Background

rSec6 and rSec8 are rat homologs of the yeast Sec6 and Sec8 proteins that are essential for protein transport between the Golgi apparatus and the plasma membrane. In yeast, Sec8 is part of a 19.5S complex that includes Sec6 and Sec15, that also associate with the ras-like GTP binding protein Sec4. An analogous 17S complex in rat brain was found to contain rSec6 and rSec8, along with eight proteins with a combined molecular weight of 743 kDa. The membrane-associated rSec6/8 complex co-immunoprecipitates with syntaxin, providing evidence that this complex is involved in synaptic vesicle docking and fusion at the presynaptic terminal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SEC6 Antibody (NBP1-97500)(6)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol EXOC3