CEP170 Antibody

Images

 
Western Blot: CEP170 Antibody [NB100-55317] - Hela whole cell lysate (5, 15, and 50 ug) stained with CEP170 Antibody diluted at 0.04 ug/ml.
Immunocytochemistry/ Immunofluorescence: CEP170 Antibody [NB100-55317] - NBF-fixed asynchronous HeLa cells, stained wtih CEP170 Antibody diluted at 1:200. Detection: Red-fluorescent goat anti-rabbit IgG highly ...read more
Immunoprecipitation: CEP170 Antibody [NB100-55317] - Hela whole cell lysate (1 mg) immunoprecipitated by rabbit anti-CEPT170 antibodies NB100-55317 at 3 ug/mg lysate.CEP170 was also immunoprecipitated by rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP, CHIP-SEQ
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

CEP170 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1534 and the C-teriminus (residue 1584) of human centrosomal protein 170kDa using the numbering given in entry NP_055627.2 (GeneID 9859).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CEP170
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Chromatin Immunoprecipitation Sequencing
Application Notes
For ICC/IF: Formaldehyde fixation with Triton-X 100 permeabilization is recommended.
Publications
Read Publication using NB100-55317.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CEP170 Antibody

  • centrosomal protein 170kDa
  • Cep170
  • KARP 1 binding protein
  • KARP-1-binding protein
  • KARP1-binding protein
  • KIAA0470FAM68AKABcentrosomal protein of 170 kDa
  • XRCC5 binding protein

Background

The product of this gene is a component of the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. During interphase, the encoded protein localizes to the sub-distal appendages of mature centrioles, which are microtubule-based structures thought to help organize centrosomes. During mitosis, the protein associates with spindle microtubules near the centrosomes. The protein interacts with and is phosphorylated by polo-like kinase 1, and functions in maintaining microtubule organization and cell morphology. The human genome contains a putative transcribed pseudogene. Several alternatively spliced transcript variants of this gene have been found, but the full-length nature of some of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CEP170 Antibody (NB100-55317)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CEP170
Entrez
Uniprot