CEP170 Antibody

Western Blot: CEP170 Antibody [NB100-55317] - Hela whole cell lysate (5, 15, and 50 ug) stained with CEP170 Antibody diluted at 0.04 ug/ml.

Immunocytochemistry/Immunofluorescence: CEP170 Antibody [NB100-55317] - NBF-fixed asynchronous HeLa cells, stained wtih CEP170 Antibody diluted at 1:200. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus(TM) 555.

Immunoprecipitation: CEP170 Antibody [NB100-55317] - Hela whole cell lysate (1 mg) immunoprecipitated by rabbit anti-CEPT170 antibodies NB100-55317 at 3 ug/mg lysate.CEP170 was also immunoprecipitated by rabbit anti-CEP170 antibodies NB100-55317 and BL4463, which recognize upstream epitopes.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP, CHIP-SEQ
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CEP170 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1534 and the C-teriminus (residue 1584) of human centrosomal protein 170kDa using the numbering given in entry NP_055627.2 (GeneID 9859).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CEP170
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Chromatin Immunoprecipitation Sequencing
• Application Notes: For ICC/IF: Formaldehyde fixation with Triton-X 100 permeabilization is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CEP170 Antibody

• centrosomal protein 170kDa
• Cep170
• KARP 1 binding protein
• KARP-1-binding protein
• KARP1-binding protein
• KIAA0470FAM68AKABcentrosomal protein of 170 kDa
• XRCC5 binding protein

Background

The product of this gene is a component of the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. During interphase, the encoded protein localizes to the sub-distal appendages of mature centrioles, which are microtubule-based structures thought to help organize centrosomes. During mitosis, the protein associates with spindle microtubules near the centrosomes. The protein interacts with and is phosphorylated by polo-like kinase 1, and functions in maintaining microtubule organization and cell morphology. The human genome contains a putative transcribed pseudogene. Several alternatively spliced transcript variants of this gene have been found, but the full-length nature of some of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEP170 Antibody (NB100-55317)(1)

Publication using NB100-55317

• Baron Gaillard CL, Pallesi-Pocachard E, Massey-Harroche D et al. Hook2 is involved in the morphogenesis of the primary cilium. Mol Biol Cell 2011 Dec [PMID: 21998199]

Bioinformatics

• Gene Symbol: CEP170
• Entrez:
  • 9859(Human)
• Uniprot:
  • Q5SW79(Human)