CENPJ Antibody

Western Blot: CENPJ Antibody [NBP1-50018] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used at 0.04 ug/ml.

• Reactivity: Hu, Pm
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CENPJ Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 675 and 725 of human Centromere Protein J using the numbering given in entry NP_060921.2 (GeneID 55835).
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CENPJ
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
• Application Notes: Immunoprecipitation Not recommended. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CENPJ Antibody

• BM032
• CENP-J
• centromere protein J
• Centrosomal P4.1-associated protein
• CPAPMCPH6
• LAG-3-associated protein
• LAPSCKL4
• LIP1microcephaly, primary autosomal recessive 6
• LYST-interacting protein 1
• LYST-interacting protein LIP1
• LYST-interacting protein LIP7
• MGC131581
• MGC131582
• MGC142222
• MGC142224
• Sas-4
• SASS4

Background

This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CENPJ