CENPA Antibody (5A7-2E11) (NBP2-12892)

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Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892] - analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11. Primary Antibody: Mouse ...read more
Immunocytochemistry/ Immunofluorescence: CENPA Antibody (5A7-2E11) [NBP2-12892] - Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CENP-A ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
5A7-2E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CENPA Antibody (5A7-2E11) Summary

Immunogen
Synthetic peptide corresponding to a portion of human CENP-A
Localization
Nucleus, Chromosome, Centromere, Kinetochore
Marker
Centromeres Marker
Specificity
Detects approx 18kDa. Recognizes Human CENP-A.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CENPA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence
Application Notes
1 ug/ml of CENP-A Antibody was sufficient for detection of CENPA in 20 ug of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CENPA Antibody (5A7-2E11)

  • Centromere autoantigen A
  • centromere protein A (17kD)
  • centromere protein ACENP-Acentromere protein A, 17kDa
  • histone H3-like centromeric protein A

Background

1 ug/mL was sufficient for detection of CENPA in 20ug of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CENPA
Uniprot