CEBP alpha Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

CEBP alpha Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human CEBP α
  Met1-Ala124
  Accession # P49715
• Specificity: Detects human CEBP alpha in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CEBP beta , rhCEBP gamma , rhCEBP δ, rhCEBP zeta , and rhCEBP epsilon is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEBP alpha Antibody [Alexa Fluor® 405]

• C/EBP alpha
• C/EBP-alpha
• CCAAT/enhancer binding protein (C/EBP), alpha
• CCAAT/enhancer-binding protein alpha
• CEBP alpha
• CEBP
• CEBPA

Background

CEBP alpha (CCAAT/Enhancer-Binding Protein; also C/EBP) is a 42-43 kDa member of the C/EBP subfamily, bZIP family of proteins. It is expressed in multiple cell types, particularly those that are fully differentiated, and includes hepatocytes, skeletal muscle cells, adipocytes, intestinal epithelium and proliferating macrophages. CEBP alpha acts as a transcriptional activator for a large and diverse number of genes, including glycogen synthase, GLUT4, and G-CSFR, and participates in both cell cycle progression and differentiation. Human CEBP alpha  is 358 amino acids (aa) in length). It contains one transactivation domain (aa 1-96), poly-Gly segment (aa 99-104), a poly-Pro motif (aa 183-189), a bZIP dimerization region (aa 281-334), and an overlapping DNA-binding Leu-zipper domain (aa 317-345). CEBP alpha  forms both homodimers, and heterodimers with other subfamily members. One alternative start site at Met120 generates a low activity 30-32 kDa isoform variant. There is also a utilized phosphorylation site at Ser21 that regulates activity. Over aa 1-124, human CEBP alpha shares 93% aa identity with mouse CEBP alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.