CEBP Delta Antibody

Images

 
Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of ...read more
Western Blot: CEBP Delta Antibody [NB110-85519] - Western blot using NB110-85519 shows detection of endogenous protein in whole cell extracts from mouse macrophage cells. The blot shows staining as follows: untreated WT ...read more
Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 ug per lane Primary: Anti-C/EBPd ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.33 mg/ml

Order Details

CEBP Delta Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse CEBP.
Specificity
This antibody recognizes endogenous mouse and recombinant human C/EBP delta protein. A BLAST analysis was used to suggest cross-reactivity with C/EBPd from human sources based on 77% identity with the immunizing sequence. No cross-reactivity is observed with mouse C/EBPa or C/EBPbeta. Reactivity with C/EBPd from other sources has not been determined.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CEBPD
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:15000
  • ELISA 1:4000-1:9000
  • Immunohistochemistry 1:2000
  • Immunoprecipitation 0.8-1.5 ug/ml
Application Notes
This affinity purified antibody has been tested for use in ELISA, western blotting, and IHC. Expect a band approximately 35 kDa in size corresponding - C/EBPd by western blotting in the appropriate cell lysate or extract. The predicted molecular weight of mouse C/EBPd is 28.8 kDa. This antibody is capable of detecting both over-expressed and endogenous C/EBPd.
Publications
Read Publications using
NB110-85519 in the following applications:

  • WB
    1 publication

Reactivity Notes

No cross-reactivity is observed with mouse CEBP Alpha or CEBP Beta. Reactivity with CEBP Delta from other sources has not been determined

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.33 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CEBP Delta Antibody

  • C/EBP-delta
  • CCAAT/enhancer binding protein (C/EBP), delta
  • CCAAT/enhancer-binding protein delta
  • CELF
  • CRP3
  • NF-IL6-betaC/EBP delta
  • Nuclear factor NF-IL6-beta

Background

C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression. Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T). Phosphorylation of C/EBP delta may increase binding activity, whereas phosphorylation of the alpha and beta family members may decrease binding affinity. C/EBP delta is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CEBP Delta Antibody (NB110-85519)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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(1)
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(2)
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Bioinformatics

Gene Symbol CEBPD
Entrez