CEBP Delta Antibody

Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: Human C/EBPbeta. Lane 2: Mouse C/EBPdelta. Lane 3: untransfected. Lane 4: Human C/EBPdelta. 35 ug per lane. Primary: Anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, 35 kDa.

Western Blot: CEBP Delta Antibody [NB110-85519] - Detection of endogenous protein in whole cell extracts from mouse macrophage cells. Untreated WT cells (Lane 1), WT cells treated 3 hrs with LPS (Lane 2) and knock-out cells treated 3 hrs with LPS (Lane 3). The band at ~35 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of endogenous C/EBP delta. Primary antibody at 1:15,000. ECL was used for detection.

Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: untreated wild-type (WT) cells. Lane 2: WT cells treated 3 hrs with LPS. Lane 3: C/EBPd-knock-out cells treated 3 hrs with LPS. 35 ug per lane. Primary: anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, 35 kDa.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CEBP Delta Antibody Summary

• Immunogen: This affinity purified CEBP Delta Antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse CEBP Delta. (Uniprot: Q00322)
• Specificity: No cross-reactivity is observed with mouse CEBP Deltaa or CEBP Deltab.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CEBPD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:4000-1:9000
  • Immunohistochemistry 1:2000
  • Immunoprecipitation 0.8-1.5 ug/ml
  • Knockdown Validated
  • Western Blot 1:1000-1:15000
• Application Notes: Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35 kDa in size corresponding to C/EBPd by western blotting in the appropriate cell lysate or extract. The predicted molecular weight of mouse C/EBPd is 28.8 kDa. This antibody is capable of detecting both over-expressed and endogenous C/EBPd.
• Theoretical MW: 28.63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

No cross-reactivity is observed with mouse CEBP Alpha or CEBP Beta. Reactivity with CEBP Delta from other sources has not been determined

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for CEBP Delta Antibody

• C/EBP-delta
• CCAAT/enhancer binding protein (C/EBP), delta
• CCAAT/enhancer-binding protein delta
• CELF
• CRP3
• NF-IL6-betaC/EBP delta
• Nuclear factor NF-IL6-beta

Background

C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression. Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T). Phosphorylation of C/EBP delta may increase binding activity, whereas phosphorylation of the alpha and beta family members may decrease binding affinity. C/EBP delta is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEBP Delta Antibody (NB110-85519)(2)

Publications using NB110-85519

• Kim D, Nam H, Lee W et al. PKCa-LSD1-NF-kB-Signaling Cascade Is Crucial for Epigenetic Control of the Inflammatory Response Molecular Cell 2018 Jan 01 [PMID: 29395062] (Mouse)
• Wang YW, Kuo CF. 2,4,5-Trimethoxybenzaldehyde, a Bitter Principle in Plants, Suppresses Adipogenesis through the Regulation of ERK1. J. Agric. Food Chem. 2014 Sep 29 [PMID: 25222709] (WB, Mouse)

Review for CEBP Delta Antibody (NB110-85519) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Wangsheng Wang Immunocytochemistry Human 07/10/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: Fetal membranes
• Species: Human
• Lot: 21589

Bioinformatics

• Gene Symbol: CEBPD
• Entrez:
  • 1052(Human)
  • 12609(Mouse)