Home » CEACAM5/CD66e » CEACAM5/CD66e Antibodies » CEACAM5/CD66e Antibody (SPM584) [Allophycocyanin]

CEACAM5/CD66e Antibody (SPM584) [Allophycocyanin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
SPM584
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34851AF350
Alexa Fluor 405
NBP2-34851AF405
Alexa Fluor 488
NBP2-34851AF488
Alexa Fluor 532
NBP2-34851AF532
Alexa Fluor 594
NBP2-34851AF594
Alexa Fluor 647
NBP2-34851AF647
Alexa Fluor 700
NBP2-34851AF700
Alexa Fluor 750
NBP2-34851AF750
Allophycocyanin/Cy7
NBP2-34851APCCY7
Biotin
NBP2-34851B
DyLight 350
NBP2-34851UV
DyLight 405
NBP2-34851V
DyLight 488
NBP2-34851G
DyLight 550
NBP2-34851R
DyLight 594
NBP2-34851DL594
DyLight 650
NBP2-34851C
DyLight 680
NBP2-34851FR
DyLight 755
NBP2-34851IR
FITC
NBP2-34851F
HRP
NBP2-34851H
Janelia Fluor 549
NBP2-34851JF549
Janelia Fluor 646
NBP2-34851JF646
PE
NBP2-34851PE
PE/Atto594
NBP2-34851PEATT594
PE/Cy5.5
NBP2-34851PECY55
PE/Cy7
NBP2-34851PECY7
PerCP
NBP2-34851PCP
Unconjugated
NBP2-33009-0.1mg
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-33009-0.1mg
Azide and BSA Free
NBP2-34851-0.1mg
IHC-Prediluted
NBP2-44896
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CEACAM5/CD66e Antibody (SPM584) [Allophycocyanin] Summary

Immunogen
Human colon carcinoma extract
Localization
Cytoplasmic and luminal surface
Specificity
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CEACAM5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CEACAM5/CD66e Antibody (SPM584) [Allophycocyanin]

  • Carcinoembryonic antigen
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • CD66e antigen
  • CD66e
  • CEA
  • CEACAM5
  • CEACAM-5
  • CEACD66e
  • DKFZp781M2392
  • Meconium antigen 100

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB120-22711
Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MUC1 [SM3] at 5 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: MUC-1 Antibody (SM3) [NB120-22711] - IHC analysis of formalin fixed paraffin embedded tissue section of human breast cancer xenograft using MUC-1 antibody clone SM3 at 1:10 dilution. The xenograft section depicted a very specific and intense signal in the periphery of the cancer cells. The necrotic cells also developed a strong immune-positivity while the tumor stroma as well as the nuclei of cells were negative for immunostaining.
Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MUC-1 antibody was tested in MCF7 cells at 10 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.
(1 Review)
(11 Publications)
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

alpha-Fetoprotein/AFP ...
MAB1368
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and alpha ‑Fetoprotein/AFP knockout HepG2 cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) in the parental HeLacell line, but is not detectable in knockout HeLa cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
alpha -Fetoprotein/AFP knockout HepG2 hepatocellular carcinoma cell line was stained with Mouse Anti-Human alpha -Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0102B). No staining in the alpha -Fetoprotein/AFP knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
(7 Reviews)
(36 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICC, ICFlow, KO
TRIM26 Antibody
NBP2-47594
Immunocytochemistry/Immunofluorescence: TRIM26 Antibody [NBP2-47594] - Staining human cell line A-431 shows positivity in cytoplasm and nucleus but excluded from the nucleoli.
Immunohistochemistry-Paraffin: TRIM26 Antibody [NBP2-47594] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center and non-germinal center cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Enolase
2/Neuron-specific Eno ...
NB110-58870
Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of different tissue and cell lysates using rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. A single band at about 47kDa corresponds to the NSE protein, seen only in extracts containing neurons ro neuronal lineage cells.
Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of mixed cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:500 in red, and costained with chicken pAb to GFAP, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. the NSE antibody labels protein expressed in neuronal cells, while the GFAP antibody stains intermediate filaments in astrocytic and certain other glial cells.
Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa.
(2 Reviews)
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, KD

CSE1L/CAS/Exportin-2 A
H00001434-M08
Western Blot: CSE1L/CAS/Exportin-2 Antibody (3D8) [H00001434-M08] - Analysis of CSE1L expression in Hela S3 NE (Cat # L013V3).
Immunocytochemistry/Immunofluorescence: CSE1L/CAS/Exportin-2 Antibody (3D8) [H00001434-M08] - Analysis of monoclonal antibody to CSE1L on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody (3D8) [H00001434-M08] - Analysis of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml
(13 Publications)
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
p130Cas/BCAR1
Antibody (OTI1A9)
NBP2-00532
Western Blot: BCAR1 Antibody (1A9) [NBP2-00532] Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.
Immunocytochemistry/Immunofluorescence: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1.
Immunohistochemistry-Paraffin: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BCAR1 mouse monoclonal antibody.
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
p120-catenin
Antibody
NBP1-85383
Western Blot: p120-catenin Antibody [NBP1-85383] - Analysis using Anti-CTNND1 antibody NBP1-85383 (A) shows similar pattern to independent antibody NBP2-38130 (B).
Immunocytochemistry/Immunofluorescence: p120-catenin Antibody [NBP1-85383] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: p120-catenin Antibody [NBP1-85383] - Staining of human small intestine shows cytoplasmic and membranous positivity in glandular cells.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Cytokeratin 19
Antibody
NB100-687
Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under standard reducing conditions using the 12-230 kDa separation system.
Western Blot: Cytokeratin 19 Antibody [NB100-687] - Western blot detection of YAP and TAZ in proteins extracted from isolated hepatocytes and cholangiocytes and total liver tissue. Antibodies against CK19 and HNF4a were used to confirm purity of the populations and b-actin was used as a loading control. Representative results from a single experiment with n = 3 independent cell isolations and n = 2 total liver tissues. Image collected and cropped by CiteAb from the following publication(http://embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - TUDCA induces intermediate states of mesenchymal and epithelial cells in primary cultures of rat HSC during hepatic differentiation. Under control conditions HSC remained negative for K19 (Cytokeratin 19) expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 uM TUDCA for 14 days (red). (f) K19 was found to be co-expressed with desmin (green), which indicated the origin of epithelial progenitor cells from HSC. (I) Cells with different states of maturation were still found after 21 days of TUDCA treatment. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep13320), licensed under a CC-BY licence.
(1 Review)
(6 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
ETFA Antibody
NBP2-16382
Western Blot: ETFA Antibody [NBP2-16382] - Wild-type (WT) and ETFA knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: ETFA Antibody [NBP2-16382] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using ETFA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ETFA antibody dilution: 1:500.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
Cytokeratin 20
Antibody (SA35-03)
NBP2-67309
Western Blot: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Analysis of Cytokeratin 20 on different cell lysates using anti-Cytokeratin 20 antibody at 1/1,000 dilution. Positive control: Lane 1: Lovo Lane 2: HCT116 Lane 3: CRC
Immunocytochemistry/Immunofluorescence: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Staining Cytokeratin 20 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Analysis of paraffin-embedded rat small intestine tissue using anti-Cytokeratin 20 antibody. Counter stained with hematoxylin.
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
CA125/MUC16
Antibody (X75)
NB600-1468
CA125/MUC16 Antibody (X75)
(3 Publications)
Species: Hu
Applications: WB, S-ELISA
Vimentin Antibody
NB300-223
Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Comparative analysis of epithelial and mesenchymal markers expressions. Fluorescence immunocytochemistry staining for E-cadherin (green), N-cadherin (red) and vimentin (cyan) was performed on esophageal cancer cells under static and microfluidic flow conditions at different time points. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep38221), licensed under a CC-BY licence.
Immunohistochemistry: Vimentin Antibody [NB300-223] - Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. Confocal microscopy images of dorsal and ventral regions of SVZ in G93A-SOD1 and Wt-SOD1 brain at postnatal week 18, stained for nestin (green), GFAP (red), and vimentin (blue). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (http://www.molecularbrain.com/content/8/1/5), licensed under a CC-BY licence.
(6 Reviews)
(45 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
CEACAM6/CD66c
Antibody (1H7-4B)
NBP2-54626
Immunohistochemistry: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Analysis in human lung: Using Anti-CEACAM6 [1H7-4B] (10 ug/ml) developed with DAB-staining procedure. Dark line indicated shows CEACAM6 expression.
Flow Cytometry: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM6 [1H7-4B] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).
ELISA: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Solid phase was coated with 3 ug/ml Anti-CEA binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM6 [1H7-4B] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.
(1 Publication)
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IP, CyTOF-ready
p53 Antibody (PAb
240)
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
(3 Reviews)
(28 Publications)
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
Kallikrein 3/PSA
[HRP]
DKK300
(1 Review)
(13 Publications)
Species: Hu
Procalcitonin
Antibody (44D9)
NB120-14817
Procalcitonin Antibody (44D9)
(1 Review)
(6 Publications)
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, S-ELISA
Serpin B3/SCCA1
Antibody (OTI1A12)
NBP2-45788
Western Blot: Serpin B3/SCCA1 Antibody (1A12) [NBP2-45788] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Serpin B3/SCCA1.
Immunohistochemistry: Serpin B3/SCCA1 Antibody (1A12) [NBP2-45788] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Serpin B3/SCCA1 Antibody (1A12) [NBP2-45788] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Species: Hu
Applications: WB, IHC, IHC-P
Cytokeratin 7
Antibody (OV-TL12/30) ...
NBP2-47940
Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).
Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)
(1 Review)
Species: Hu, Mu
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, IF

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34851AF350
Alexa Fluor 405 NBP2-34851AF405
Alexa Fluor 488 NBP2-34851AF488
Alexa Fluor 532 NBP2-34851AF532
Alexa Fluor 594 NBP2-34851AF594
Alexa Fluor 647 NBP2-34851AF647
Alexa Fluor 700 NBP2-34851AF700
Alexa Fluor 750 NBP2-34851AF750
Allophycocyanin NBP2-34851APC
Allophycocyanin/Cy7 NBP2-34851APCCY7
Biotin NBP2-34851B
DyLight 350 NBP2-34851UV
DyLight 405 NBP2-34851V
DyLight 488 NBP2-34851G
DyLight 550 NBP2-34851R
DyLight 594 NBP2-34851DL594
DyLight 650 NBP2-34851C
DyLight 680 NBP2-34851FR
DyLight 755 NBP2-34851IR
FITC NBP2-34851F
HRP NBP2-34851H
Janelia Fluor 549 NBP2-34851JF549
Janelia Fluor 646 NBP2-34851JF646
PE NBP2-34851PE
PE/Atto594 NBP2-34851PEATT594
PE/Cy5.5 NBP2-34851PECY55
PE/Cy7 NBP2-34851PECY7
PerCP NBP2-34851PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol CEACAM5