CEACAM5/CD66e Antibody (SPM330)

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Immunohistochemistry-Paraffin: CEACAM-5/CD66e Antibody (SPM330) [NBP2-44898] - Human Colon Carcinoma stained with CEA Monoclonal Antibody (SPM330).

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications Flow, IHC-P, ICC/IF
Clone
SPM330
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

CEACAM5/CD66e Antibody (SPM330) Summary

Immunogen
Purified human CEA protein
Localization
Cytoplasmic and luminal surface
Specificity
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb reacts with nonspecific cross-reacting antigen (NCA) and shows a cross-reaction with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CEACAM5
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
Application Notes
Hu-chromosome location: 19q13.1-19q13.2
Molecular weight of antigen: 80-200kDa
Immunohistochemistry-Paraffin 1 - 2 ug/ml for 30 minutes at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for CEACAM5/CD66e Antibody (SPM330)

  • Carcinoembryonic antigen
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • CD66e antigen
  • CD66e
  • CEA
  • CEACAM5
  • CEACAM-5
  • CEACD66e
  • DKFZp781M2392
  • Meconium antigen 100

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CEACAM5
Uniprot