CEACAM5/CD66e Antibody (COL-1 + CEA31 + C66/261) - Azide and BSA Free

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Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (COL-1 + CEA31 + C66/261) - Azide and BSA Free [NBP2-34597] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with CEA MAb (COL-1+CEA31+C66/261)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
COL-1 + CEA31 + C66/261
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

CEACAM5/CD66e Antibody (COL-1 + CEA31 + C66/261) - Azide and BSA Free Summary

Immunogen
Human colon carcinoma extract (COL-1 & CEA31); CEA recombinant protein (C66/261)
Localization
Cytoplasmic and luminal surface
Specificity
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
Isotype
IgG2a/IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CEACAM5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25-0.5ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1-2ug/ml
  • Immunohistochemistry-Paraffin 1-2ug/ml
  • Immunoprecipitation 1-2ug/500ug protein
  • CyTOF-ready
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CEACAM5/CD66e Antibody (COL-1 + CEA31 + C66/261) - Azide and BSA Free

  • Carcinoembryonic antigen
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • CD66e antigen
  • CD66e
  • CEA
  • CEACAM5
  • CEACAM-5
  • CEACD66e
  • DKFZp781M2392
  • Meconium antigen 100

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CEACAM5/CD66e Antibody (NBP2-34597) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34597AF350
Alexa Fluor 405 NBP2-34597AF405
Alexa Fluor 488 NBP2-34597AF488
Alexa Fluor 532 NBP2-34597AF532
Alexa Fluor 594 NBP2-34597AF594
Alexa Fluor 647 NBP2-34597AF647
Alexa Fluor 700 NBP2-34597AF700
Alexa Fluor 750 NBP2-34597AF750
Allophycocyanin NBP2-34597APC
Allophycocyanin/Cy7 NBP2-34597APCCY7
Biotin NBP2-34597B
DyLight 350 NBP2-34597UV
DyLight 405 NBP2-34597V
DyLight 488 NBP2-34597G
DyLight 550 NBP2-34597R
DyLight 594 NBP2-34597DL594
DyLight 650 NBP2-34597C
DyLight 680 NBP2-34597FR
DyLight 755 NBP2-34597IR
FITC NBP2-34597F
HRP NBP2-34597H
Janelia Fluor 549 NBP2-34597JF549
Janelia Fluor 646 NBP2-34597JF646
PE NBP2-34597PE
PE/Atto594 NBP2-34597PEATT594
PE/Cy5.5 NBP2-34597PECY55
PE/Cy7 NBP2-34597PECY7

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Bioinformatics

Gene Symbol CEACAM5