CEACAM5/CD66e Antibody (C66/261)

Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (C66/261) [NBP2-33010] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (C66/261).

Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (C66/261) [NBP2-33010] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (C66/261).

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC
• Clone: C66/261
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CEACAM5/CD66e Antibody (C66/261) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34586)
  
  Antibody with azide - store at 2 to 8C.
• Immunogen: Recombinant full-length human CEACAM5/CD66e protein (Uniprot: P06731)
• Localization: Cytoplasmic and luminal surface
• Specificity: This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This monoclonal antibody does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbladder, urachus, salivary gland, ovary, and endocervix.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEACAM5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 0.5-1 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CEACAM5/CD66e Antibody (C66/261)

• Carcinoembryonic antigen
• carcinoembryonic antigen-related cell adhesion molecule 5
• CD66e antigen
• CD66e
• CEA
• CEACAM5
• CEACAM-5
• CEACD66e
• DKFZp781M2392
• Meconium antigen 100

Background

The CD66e (CEA; 180-200 kDa) is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion. Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas - potential target of tumor imaging and drug targeting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CEACAM5/CD66e Antibody (NBP2-33010). (Showing 1 - 1 of 1 FAQ).

1. I recently ordered several antibodies (Cat No. NBP2-33010, NBP2-33011, NBP2-33012) to run in a FACS experiment. What concentration are the 0.5ml vials? It’s not listed on the vial, and I would like to start with the recommended FACS dilution (0.5-1ug/million cells) listed on the datasheet.
   • All of these three monoclonal antibodies have the concentration of 200 ug/ml. Therefore please use 2.5 - 5 ul of each antibody solution per 1 million cells tested in flow. 

Bioinformatics

• Gene Symbol: CEACAM5
• Uniprot:
  • P06731()
  • P06731()
  • P06731()