CEACAM5/CD66e Antibody (5C8C4) [Alexa Fluor® 488]

Flow Cytometry: CEACAM5/CD66e Antibody (5C8C4) [Alexa Fluor® 488] [NBP2-54625AF488] - SKCO1 and MCF7 (breast cancer cell line) cells. Image from verified customer review.

Flow Cytometry: CEACAM5/CD66e Antibody (5C8C4) [Alexa Fluor® 488] [NBP2-54625AF488] - Analysis of human HPAC cells expressing ceacam5 (red). Mouse isotype control (yellow). Image from verified customer review.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, IP, CyTOF-ready
• Clone: 5C8C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

CEACAM5/CD66e Antibody (5C8C4) [Alexa Fluor® 488] Summary

• Immunogen: Soluble human CEACAM5/CD66e
• Specificity: This antibody recognizes human CEACAM 5. It does not cross-react with CEACAM-1, -3, -4, -6, -7, and -8 or rat CEACAM1, mouse CEACAM1 and mouse CEACAM2.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEACAM5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CEACAM5/CD66e Antibody (5C8C4) [Alexa Fluor® 488]

• Carcinoembryonic antigen
• carcinoembryonic antigen-related cell adhesion molecule 5
• CD66e antigen
• CD66e
• CEA
• CEACAM5
• CEACAM-5
• CEACD66e
• DKFZp781M2392
• Meconium antigen 100

Background

The CD66e (CEA; 180-200 kDa) is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion. Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas - potential target of tumor imaging and drug targeting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for CEACAM5/CD66e Antibody (NBP2-54625AF488) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 12/17/2018

Summary

• Application: Flow Cytometry
• Sample Tested: breast cancer MCF7 cells,SKCO1
• Species: Human
• Lot: CRT/17/49-01318-AF488

Comments

• Comments: Harvested cells and aliquoted up to 1 x 106 cells/100 μL into FACS tubes. Fc-block cells with blocking IgG (1 μg IgG/106 cells) for 15 minutes at room temperature.
  Added conjugated antibody and vortex. Incubate cells for 45-60min minutes at 2-4C
  Wash 3X
  Resuspend the cells in 200 – 400 μL of Flow Cytometry Staining Buffer for final flow cytometric analysis

reviewed by: Verified Customer Flow Human 10/27/2018

Summary

• Application: Flow Cytometry
• Sample Tested: HPAC cell line
• Species: Human
• Lot: CRT/17/49-01318-AF488

Bioinformatics

• Gene Symbol: CEACAM5