CDX2 Antibody (EPR2764Y)

Product Discontinued

CDX2 Antibody (EPR2764Y) Summary

• Immunogen: A synthetic peptide corresponding to residues near the N-terminus of human CDX-2 was used as an immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDX2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:40
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-500
  • Simple Western 1:100
  • Western Blot 1:1000-5000
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26812015).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Culture supernatant in buffered aqueous solution
• Preservative: No Preservative
• Concentration: .022 mg/ml
• Purity: Protein A or G purified

Notes

This is a high affinity, high titer antibody. Optimal dilutions should be determined by the end user. (Provided dilutions serve only as a suggestive starting point.)

Alternate Names for CDX2 Antibody (EPR2764Y)

• caudal type homeo box transcription factor 2
• caudal type homeobox 2
• Caudal-type homeobox protein 2
• CDX2
• CDX3
• CDX-3
• CDX3caudal type homeobox transcription factor 2
• homeobox protein CDX-2

Background

CDX2 is a caudal type homeobox gene that encodes an intestine-specific transcription factor that is expressed early in intestinal development and may be involved in the regulation of proliferation and differentiation of intestinal epithelial cells. It is expressed in the nuclei of epithelial cells throughout the intestine, from duodenum to rectum. The CDX2 protein is expressed in primary and metastatic colorectal carcinomas and has also been demonstrated in the intestinal metaplasia of the stomach and intestinal-type gastric cancer, while it is not expressed in the normal gastric mucosa. Studies have shown that CDX2 is superior marker compared to CK20.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDX2 Antibody (NBP1-40553)(4)

Publications using NBP1-40553

• Workman MJ, Gleeson JP, Troisi EJ et al. Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips Cell Mol Gastroenterol Hepatol 2017-12-29 [PMID: 29930984] (IF/IHC, Human)
• Matsubara D, Soda M, Yoshimoto T et al. Inactivating mutations and hypermethylation of the NKX2-1/TTF-1 gene in non-terminal respiratory unit-type lung adenocarcinomas Cancer Sci. 2017-09-01 [PMID: 28677170] (IHC-P, Human)
• Branco MR, King M, Perez-Garcia V et al. Maternal DNA Methylation Regulates Early Trophoblast Development. Dev. Cell. 2016-01-25 [PMID: 26812015] (IHC-P, Mouse)
• Home P, Ray S, Dutta D et al. GATA3 is selectively expressed in the trophectoderm of peri-implantation embryo and directly regulates Cdx2 gene expression. J Biol Chem. 2009-10-01 [PMID: 19700764]

Bioinformatics

• Gene Symbol: CDX2
• Entrez:
  • 1045(Human)
• Uniprot:
  • Q99626()