TTF-1 / NKX2-1 Antibody (OTI10F8)

Images

 
Western Blot: TTF-1 / NKX2-1 Antibody (OTI10F8) [NBP2-46175] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NKX2-1.
Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (OTI10F8) [NBP2-46175] - Staining of NCI-H1975 cells using anti-NKX2-1 mouse monoclonal antibody . The right is A549 cells as negative control.
Immunohistochemistry: TTF-1 / NKX2-1 Antibody (OTI10F8) [NBP2-46175] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TTF-1 / NKX2-1 Antibody (OTI10F8) [NBP2-46175] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (OTI10F8) [NBP2-46175] - Staining of TT cells using anti-NKX2-1 mouse monoclonal antibody . The right is A549 cells as negative control.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
OTI10F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

TTF-1 / NKX2-1 Antibody (OTI10F8) Summary

Immunogen
Full length human recombinant protein of human NKX2-1(NP_003308) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NKX2-1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot
Theoretical MW
38.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TTF-1 / NKX2-1 Antibody (OTI10F8)

  • BCH
  • benign chorea
  • BHC
  • Homeobox protein NK-2 homolog A
  • homeobox protein Nkx-2.1
  • NK2 homeobox 1
  • NK-2 homolog A
  • NKX2.1
  • NKX2-1
  • NKX2A
  • NKX2ANKX2.1
  • T/EBP
  • Thyroid nuclear factor 1
  • Thyroid transcription factor 1BHC
  • TITF1
  • TITF1TEBP
  • TTF1
  • TTF-1
  • TTF1BCH
  • TTF-1NK-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NKX2-1