CDP/CUTL1 Antibody

Product Discontinued

CDP/CUTL1 Antibody Summary

• Immunogen: This antibody is specific for the C Terminus Region of the target protein.
• Specificity: This product is specific for Human CUTL1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CUX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 1:100 - 1:2000
  • Western Blot 1:5000 - 1:20000

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Potassium Phosphate (pH 7.0), 0.15 M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for CDP/CUTL1 Antibody

• CASP
• CCAAT displacement protein
• CDP
• CDP/Cux
• CDPCCAAT displacement protein
• COY1
• cut (Drosophila)-like 1 (CCAAT displacement protein)
• CUT
• CUTL1
• cut-like 1, CCAAT displacement protein (Drosophila)
• cut-like homeobox 1
• CUX
• CUX1
• FLJ31745
• golgi integral membrane protein 6
• GOLIM6
• homeobox protein cut-like 1
• Homeobox protein cux-1
• Nbla10317
• p100
• p110
• p200
• p75
• protein CASP
• putative protein product of Nbla10317

Background

The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDP/CUTL1 Antibody (35090002)(2)

Publications using 35090002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Review for CDP/CUTL1 Antibody (35090002) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Christopher Cardinale ChIP Human 07/06/2018

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: sheared chromatin from Jurkat cells,Sheared cross-linked chromatin
• Species: Human
• Lot: T01190A01

Comments

• Comments: Jurkat T cell acute lymphoblastic leukemia (T-ALL) cells were grown to late log phase, at a density of 850,000 cells per ml. Sheared chromatin was prepared from 30 million cells using the Covaris TruChIP kit and the Covaris LE220 sonicator according to the manufacturer's instructions. Sonication was performed at a 30% duty cycle for 15 min.
  The chromatin was precleared with MagnaChIP Protein A + G magnetic beads (Millipore) and 10 micrograms of pure rabbit normal IgG (Jackson Immunoresearch).
  The input was divided into 4 IPs of about 7 million cells each. Normal rabbit IgG (Jackson) and the 3 indicated Novus antibodies were used to IP overnight with MagnaChIP beads. The next day the beads were washed with low salt buffer, high salt buffer, LiCl buffer, and TE. The recovered DNA was eluted and purified with the iPure v2 kit (Diagenode). The recovered DNA was diluted 1:10 and 5 microliters used in a 10 microliter qPCR reaction with the QIAGEN QuantiNova SYBR Green kit.
  Amplicons were from the promoters of 5 genes: INS, ALAS1, HES1, RPA3, and JAK2.

Bioinformatics

• Gene Symbol: CUX1
• Uniprot:
  • Q13948(Human)