Cdk9 Antibody (SD204-07)

Simple Western: Cdk9 Antibody (SD204-07) [NBP2-67811] - Simple Western Wes data showing knockdown of both CDK9 isoforms using CDK9 antibody (# NBP2-67811) after THAL SNS 032 (Tocris Cat. No. 6532) treatment of MOLT-4 cells (4 h incubation). GAPDH (# AF5718) was used as a loading control. Protein quantification (relative to DMSO-only control) is shown beneath the corresponding lane.

Western Blot: Cdk9 Antibody (SD204-07) [NBP2-67811] - Western blot analysis of Cdk9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb

Immunocytochemistry/Immunofluorescence: Cdk9 Antibody (SD204-07) [NBP2-67811] - Staining Cdk9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunohistochemistry-Paraffin: Cdk9 Antibody (SD204-07) [NBP2-67811] - Analysis of paraffin-embedded human tonsil tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.

Western Blot: Cdk9 Antibody (SD204-07) [NBP2-67811] - Analysis of Cdk9 on different lysates using anti-Cdk9 antibody at 1:1,000 dilution. Positive control: Lane 1: HeLa Lane 2: Jurkat Lane 3: A431

Western Blot: Cdk9 Antibody (SD204-07) [NBP2-67811] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, 4T1, B16-F10, Hep3b and 293T cells lysates (20 ug/lane). 10% SDS-PAGE. CDK9 (NB2-67811) primary antibody at 1:2000, 4C, overnight. Image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: Cdk9 Antibody (SD204-07) [NBP2-67811] - Staining Cdk9 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunocytochemistry/Immunofluorescence: Cdk9 Antibody (SD204-07) [NBP2-67811] - Staining Cdk9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunohistochemistry-Paraffin: Cdk9 Antibody (SD204-07) [NBP2-67811] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cdk9 Antibody (SD204-07) [NBP2-67811] - Analysis of paraffin-embedded human colon cancer tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cdk9 Antibody (SD204-07) [NBP2-67811] - Analysis of paraffin-embedded human lung cancer tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.

Product Discontinued

Cdk9 Antibody (SD204-07) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Cdk9 aa 323-372 / 372. (SwissProt: P50750 Human)
• Localization: Nucleus, PML body, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDK9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Simple Western
  • Western Blot 1:500-1:1000
• Application Notes: Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:35031618)

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cdk9 Antibody (SD204-07)

• C-2K
• CDC2L4
• CDC2L4CTK1
• CDC2-related kinase
• Cdk9
• Cell division cycle 2-like protein kinase 4
• Cell division protein kinase 9
• cyclin-dependent kinase 9 (CDC2-related kinase)
• cyclin-dependent kinase 9
• EC 2.7.11
• EC 2.7.11.22
• EC 2.7.11.23
• PITALRE
• serine/threonine protein kinase PITALRE
• Serine/threonine-protein kinase PITALRE
• TAK

Background

CDK9 (PITALRE) (also known as cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K and Cell division cycle 2-like protein kinase 4) is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. CDK9 (PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. This kinase also was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. CDK9 (PITALRE) is a ubiquitously expressed nuclear protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cdk9 Antibody (NBP2-67811)(1)

Publication using NBP2-67811

• Yang Y, Lu H, Chen C et al. HIF-1 Interacts with TRIM28 and DNA-PK to release paused RNA polymerase II and activate target gene transcription in response to hypoxia Nature communications 2022-01-14 [PMID: 35031618] (Chemotaxis, WB)

Review for Cdk9 Antibody (NBP2-67811) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human and Mouse.

reviewed by: Yongkang Yang WB Human and Mouse 10/10/2020

Summary

• Application: Western Blot
• Sample Tested: cell line, whole cell lysate
• Species: Human and Mouse
• Lot: HJ0910

Bioinformatics

• Gene Symbol: CDK9