Cdk9 Antibody (SD204-07)

Images

 
Biological Strategies: Simple Western: Cdk9 Antibody (SD204-07) [NBP2-67811] - Simple Western Wes data showing knockdown of both CDK9 isoforms using CDK9 antibody (# NBP2-67811) after THAL SNS 032 (Tocris Cat. ...read more
Western Blot: Cdk9 Antibody (SD204-07) [NBP2-67811] - Western blot analysis of Cdk9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The ...read more
Immunocytochemistry/ Immunofluorescence: Cdk9 Antibody (SD204-07) [NBP2-67811] - Staining Cdk9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: Cdk9 Antibody (SD204-07) [NBP2-67811] - Analysis of paraffin-embedded human tonsil tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.
Western Blot: Cdk9 Antibody (SD204-07) [NBP2-67811] - Analysis of Cdk9 on different lysates using anti-Cdk9 antibody at 1:1,000 dilution. Positive control: Lane 1: HeLa Lane 2: Jurkat Lane 3: A431
Western Blot: Cdk9 Antibody (SD204-07) [NBP2-67811] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, 4T1, B16-F10, Hep3b and 293T cells lysates (20 ug/lane). 10% SDS-PAGE. CDK9 (NB2-67811) primary antibody at 1:2000, ...read more
Immunocytochemistry/ Immunofluorescence: Cdk9 Antibody (SD204-07) [NBP2-67811] - Staining Cdk9 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: Cdk9 Antibody (SD204-07) [NBP2-67811] - Staining Cdk9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: Cdk9 Antibody (SD204-07) [NBP2-67811] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Cdk9 Antibody (SD204-07) [NBP2-67811] - Analysis of paraffin-embedded human colon cancer tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Cdk9 Antibody (SD204-07) [NBP2-67811] - Analysis of paraffin-embedded human lung cancer tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
Clone
SD204-07
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Cdk9 Antibody (SD204-07) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within C-terminal human Cdk9. (SwissProt: P50750 Human)
Localization
Nucleus, PML body, Cytoplasm.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CDK9
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Simple Western
  • Western Blot 1:500-1:1000
Application Notes
Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:35031618)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-67811 in the following applications:

Publications
Read Publication using NBP2-67811.

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH 7.4), 1% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Cdk9 Antibody (SD204-07)

  • C-2K
  • CDC2L4
  • CDC2L4CTK1
  • CDC2-related kinase
  • Cdk9
  • Cell division cycle 2-like protein kinase 4
  • Cell division protein kinase 9
  • cyclin-dependent kinase 9 (CDC2-related kinase)
  • cyclin-dependent kinase 9
  • EC 2.7.11
  • EC 2.7.11.22
  • EC 2.7.11.23
  • PITALRE
  • serine/threonine protein kinase PITALRE
  • Serine/threonine-protein kinase PITALRE
  • TAK

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP

Publications for Cdk9 Antibody (NBP2-67811)(1)

We have publications tested in 2 applications: ChIP, WB.


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ChIP
(1)
WB
(1)
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Review for Cdk9 Antibody (NBP2-67811) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human and Mouse.

Reviews using NBP2-67811:
Filter by Applications
Western Blot
(1)
All Applications
Filter by Species
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Cdk9 NBP2-67811
Enlarge
5
reviewed by:
Verified Customer
Western Blot Human and Mouse 10/10/2020
View

Summary

ApplicationWestern Blot
Sample Testedcell line, whole cell lysate
SpeciesHuman and Mouse
LotHJ0910

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Cdk9 Antibody (NBP2-67811) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
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2
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1
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Verified Customer
10/10/2020
Application: Western Blot
Species: Human and Mouse

Bioinformatics

Gene Symbol CDK9