Cdk9 Antibody (2D7) - Azide and BSA Free

Western Blot: Cdk9 Antibody (2D7) [H00001025-M07] - Analysis of CDK9 expression in transfected 293T cell line by CDK9 monoclonal antibody (M07), clone 2D7. Lane 1: CDK9 transfected lysatE (42.778 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Cdk9 Antibody (2D7) [H00001025-M07] - Analysis of monoclonal antibody to CDK9 on HeLa cell. Antibody concentration 10 ug/ml

ELISA: Cdk9 Antibody (2D7) [H00001025-M07] - Detection limit for recombinant GST tagged CDK9 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Dr
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 2D7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Cdk9 Antibody (2D7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CDK9 (AAH01968, 271 a.a. ~ 372 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QKRKVKDRLKAYVRDPYALDLIDKLLVLDPAQRIDSDDALNHDFFWSDPMPSDLKGMLSTHLTSMFEYLAPPRRKGSQITQQSTNQSRNPATTNQTEFERVF
• Specificity: CDK9 - cyclin-dependent kinase 9 (CDC2-related kinase) (2D7)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDK9
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.Use in immunoprecipitation reported in scientific literature (PMID 28754981).

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 28754981).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cdk9 Antibody (2D7) - Azide and BSA Free

• C-2K
• CDC2L4
• CDC2L4CTK1
• CDC2-related kinase
• Cdk9
• Cell division cycle 2-like protein kinase 4
• Cell division protein kinase 9
• cyclin-dependent kinase 9 (CDC2-related kinase)
• cyclin-dependent kinase 9
• EC 2.7.11
• EC 2.7.11.22
• EC 2.7.11.23
• PITALRE
• serine/threonine protein kinase PITALRE
• Serine/threonine-protein kinase PITALRE
• TAK

Background

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cdk9 Antibody (H00001025-M07)(2)

Publications using H00001025-M07

• Yang Y, Lu H, Chen C et al. HIF-1 Interacts with TRIM28 and DNA-PK to release paused RNA polymerase II and activate target gene transcription in response to hypoxia Nature communications 2022-01-14 [PMID: 35031618] (WB, IP)
• Foo LC. Cyclin-dependent kinase 9 is required for the survival of adult Drosophila melanogaster glia Sci Rep 2017-07-28 [PMID: 28754981] (IP, Drosophila)

Bioinformatics

• Gene Symbol: CDK9
• Entrez:
  • 1025(Human)
• Uniprot:
  • AAH01968(Human)