CDK2AP1 Overexpression Lysate (Native)

Images

 
Western Blot: CDK2AP1 Overexpression Lysate (Adult Normal) [NBL1-09037] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CDK2AP1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CDK2AP1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204442 and based on accession number NM_004642. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens cyclin-dependent kinase 2 associated protein 1 (CDK2AP1), mRNA.
Gene
CDK2AP1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
12.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CDK2AP1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CDK2AP1 Overexpression Lysate (Native)

  • CDK2-associated protein 1cyclin-dependent kinase 2-associated protein 1
  • CDKAP1
  • cyclin-dependent kinase 2 associated protein 1
  • Deleted in oral cancer 1
  • Deleted in oral cancer-1
  • doc-1
  • DOC1Putative oral cancer suppressor
  • DORC1
  • p12DOC-1
  • ST19

Background

The protein encoded by this gene is a specific CDK2-associated protein, which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggested the regulatory role in DNA replication during S phase of the cell cycle. A similar gene in hamster was isolated from, and functions as a growth suppressor of normal keratinocytes. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CDK2AP1 Lysate (NBL1-09037) (0)

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Bioinformatics

Gene Symbol CDK2AP1