CDK1/2/3 [p Thr14] Antibody (4I9L3)

Western Blot: CDK1/2/3 [p Thr14] Antibody (4I9L3) [NBP3-33561] - Western blot analysis of various lysates using CDK1/2/3 Rabbit mAb at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37C for 20 hours or treated by Nocodazole (100 ng/mL) at 37C for 24 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 30s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clone: 4I9L3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CDK1/2/3 [p Thr14] Antibody (4I9L3) Summary

• Immunogen: A synthetic phosphorylated peptide around T14 of human CDK1/2/3 (P06493).
  
  Sequence:
  EGTYG
• Modification: p Thr14
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CDK1/2/3 [p Thr14] Antibody (4I9L3)

• CDC2
• CDK3
• CDKN2
• Cell division protein kinase 3
• cyclin dependent kinase 1
• cyclin dependent kinase 2
• cyclin dependent kinase 3
• p33 protein kinase
• p34 protein kinase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.