CDC45L Antibody

Images

 
Western Blot: CDC45L Antibody [NBP1-32772] - Sample (30 ug of whole cell lysate)A: H12997. 5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: CDC45L Antibody [NBP1-32772] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC45L protein stained by CDC45L antibody diluted at 1:500. Blue: Hoechst ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CDC45L Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CDC45L. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDC45
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Publications
Read Publication using NBP1-32772.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDC45L Antibody

  • CDC45 cell division cycle 45-like (S. cerevisiae)
  • cell division cycle 45 homolog (S. cerevisiae)
  • cell division cycle 45-like 2
  • human CDC45
  • S.cerevisiae, homolog)-like

Background

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDC45L Antibody (NBP1-32772)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CDC45L Antibody (NBP1-32772) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDC45