CD99 Antibody (MEM-131) [FITC]

Flow Cytometry: CD99 Antibody (MEM-131) [FITC] [NB500-622] - Surface staining of human peripheral blood with anti-CD99R (MEM-131) FITC.

• Reactivity: Hu
• Applications: WB, Flow, IHC, IP
• Clone: MEM-131
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

CD99 Antibody (MEM-131) [FITC] Summary

• Additional Information: Clone MEM-131 was used by HLDA to establish CD designation.
• Immunogen: HPB-ALL human peripheral blood leukemia T-cell line
• Specificity: The antibody MEM-131 reacts with CD99R, an epitope restricted to a subset of CD99 molecule expressed on myeloid cells, NK cells and T lymphocytes. HLDA V; WS Code AS S020 HLDA V; WS Code T T-E2.02 HLDA V; WS Code T T-017
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD99
• Purity: Size Exclusion Chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Stabilizing Tris buffered saline (TBS), pH 8.0
• Preservative: 15mM Sodium Azide
• Purity: Size Exclusion Chromatography

Alternate Names for CD99 Antibody (MEM-131) [FITC]

• 12E7
• CD99 antigenY homolog
• CD99 molecule
• CD99
• E2 antigen
• HBA71
• MIC2 (monoclonal 12E7)
• MIC2
• MIC2Y
• MSK5X
• pilr-1
• PILR-L
• Protein MIC2
• surface antigen MIC2
• T-cell surface glycoprotein E2

Background

CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD99