CD99 Antibody (MEM-131)

Images

 
Flow Cytometry: CD99 Antibody (MEM-131) [NB500-403] - Analysis using the FITC conjugate of NB500-403. Staining of Human peripheral blood lymphocytes with HCD99 FITC.
Flow Cytometry: CD99 Antibody (MEM-131) [NB500-403] - Analysis using the Biotin conjugate of NB500-403. Staining of Human peripheral blood lymphocytes biotinylated HCD99, followed by Sav-PE.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P, IP
Clone
MEM-131
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Catalog# & Conjugate Size Price

CD99 Antibody (MEM-131) Summary

Immunogen
HPB-ALL human peripheral blood leukemia T-cell line
Specificity
The antibody MEM-131 reacts with CD99R, an epitope restricted to a subset of CD99 molecule expressed on myeloid cells, NK cells and T lymphocytes. HLDA V; WS Code AS S020 HLDA V; WS Code T T-E2.02 HLDA V; WS Code T T-017
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
CD99
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:50
Application Notes
Western Blot: Use under non-reducing conditions. Antibody purity is > 95% (by SDS-PAGE).

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS (approx. pH 8.0)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for CD99 Antibody (MEM-131)

  • 12E7
  • antigen identified by monoclonal antibodies 12E7, F21 and O13
  • CD99 antigenY homolog
  • CD99 molecule
  • CD99
  • E2 antigen
  • HBA71
  • MIC2 (monoclonal 12E7)
  • MIC2
  • MIC2Y
  • MSK5X
  • pilr-1
  • PILR-L
  • Protein MIC2
  • surface antigen MIC2
  • T-cell surface glycoprotein E2

Background

CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

FITC Labeled NB500-622
PE Labeled NBP1-41282

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Bioinformatics

Gene Symbol CD99
Entrez