CD90/Thy1 Antibody (3H1751)

Product Discontinued

CD90/Thy1 Antibody (3H1751) Summary

• Immunogen: Rat Thy 1 antigen
• Localization: Attached to the membrane by a GPI anchor
• Marker: Mesenchymal Cell Marker
• Specificity: Recognizes the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus 3H1751 reacts with Thy-1.1 mice e.g. AKR and FBV strains, but not Thy-1.2 mice e.g. CBA, BALB/c. Species Crossreactivity: rabbit, mouse, and guinea pig.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: THY1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100. 10 ul/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: IHC-Fr: Use at assay dependant dilution. WB: Use at assay dependent dilution.

Reactivity Notes

Cross-reacts with Mouse and Rat THY1.1 Species Crossreactivity: rabbit, mouse, and guinea pig. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Human reactivity reported in scientific literature (PMID: 28366456). Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CD90/Thy1 Antibody (3H1751)

• CD90 antigen
• CD90
• CDw90
• FLJ33325
• Thy-1 antigen
• Thy-1 cell surface antigen
• thy-1 membrane glycoprotein
• Thy-1 T-cell antigen
• Thy1

Background

The affinity of this antibody for rat Thy-1 is 3 x 10e9m-1 and for Mouse Thy-1.1 is 3 x 10e8m-1 (1). The Thy-1 antigen is found on a variety of cell types including thymocytes, neuronal cells, T-Lymphocytes (mouse), stem cells and immature B cells (rat) and connective tissue (2). The molecule is a glycoprotein with 112 amino acids which is homologous to immunoglobulin domains (3). The binding characteristics have been extensively studied (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD90/Thy1 Antibody (NB200-528)(1)

Publication using NB200-528

• Steens J, Zuk M, Benchellal M et al. In Vitro Generation of Vascular Wall-Resident Multipotent Stem Cells of Mesenchymal Nature from Murine Induced Pluripotent Stem Cells. Stem Cell Reports. 2017-04-11 [PMID: 28366456] (FLOW, Human)

FAQs for CD90/Thy1 Antibody (NB200-528). (Showing 1 - 1 of 1 FAQ).

1. We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • I would recommend one of theses <a href="http://www.novusbio.com/productsearch/CD90%252FThy1#fq=common_name%3A%22CD90%2FThy1%22&fq=species%3A%22Canine%22" target="_self">CD90 antibodies</a>, as these have all been validated for use with dog/canine samples.

Bioinformatics

• Gene Symbol: THY1
• Entrez:
  • 21838(Mouse)
  • 24832(Rat)
• Uniprot:
  • P01830(Rat)