CD74 Antibody (PIN.1) - Azide and BSA Free

Western Blot: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657] - Western blot of CD74 from IP N87 lysates mixed with macrophage migration inhibitory factor (MIF). Image from the standard format of this antibody.

Immunocytochemistry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human renal cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human spleen using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration. Image from the standard format of this antibody.

Flow Cytometry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657] - CD74 in human PBMCs (gated on all monocytes). Orange represents isotype control and blue represents anti-CD74 antibody staining. Image from the standard format of this antibody.

Western Blot: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657] - Ramos whole cell protein was separated on a 4-12% gel by SDS-PAGE and protein transferred to PVDF. The membrane was probed with anti-CD74 at 2 ug/ml and detected with and anti-mouse HRP secondary antibody using chemiluminescence. The arrow head shows the detection of CD74. Image from the standard format of this antibody.

Immunohistochemistry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human pulmonary squamous cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration. Image from the standard format of this antibody.

• Reactivity: Hu, Mu(-)
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: PIN.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD74 Antibody (PIN.1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CD74 Antibody (PIN.1) - Azide and BSA Free (NB100-1985) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide corresponding to residues 12-27 of CD74, human invariant chain (short form).
• Epitope: The epitope is in a region of the cytoplasmic tail of human CD74 which is common to all isoforms.
• Localization: Type II membrane protein
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD74
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1 ug/ml
• Application Notes: In Simple Western only 10-15 ul of the recommended dilution is used per data point. In WB this detects an ~33-35 kDa protein doublet, corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74. This antibody is CyTOF ready.

Reactivity Notes

Human. Does not react with mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CD74 Antibody (PIN.1) - Azide and BSA Free

• CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated)
• CD74 antigen
• CD74 molecule, major histocompatibility complex, class II invariant chain
• CD74
• CLIP
• DHLAG
• DHLAGgamma chain of class II antigens
• HLA class II histocompatibility antigen gamma chain
• HLADG
• HLA-DR antigens-associated invariant chain
• HLA-DR-gamma
• Ia antigen-associated invariant chain
• Ia-associated invariant chain
• Ia-gamma
• Ii
• INVG34
• MHC HLA-DR gamma chain
• p33

Background

CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of or in association with MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds MHC class II molecules which have lost their antigenic peptide leading to internalization and reloading with antigenic peptide. The transport route of class II alpha/beta/Ii complexes is regulated selectively by two forms of CD74 (p33 and p35) which are generated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages and monocytes together, with many epithelial cells. CD74 may exist in different isoforms ranging in size from 33 to 41kDa, depending on genetic splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD74