CD69 Overexpression Lysate (Native)

Images

 
Western Blot: CD69 Overexpression Lysate (Adult Normal) [NBL1-08963] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD69.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CD69 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202756 and based on accession number NM_001781. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens CD69 molecule (CD69), transcript variant 1, mRNA.
Gene
CD69

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
22.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CD69

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CD69 Overexpression Lysate (Native)

  • activation inducer molecule (AIM/CD69)
  • Activation inducer molecule
  • AIM
  • BL-AC/P26
  • CD69 antigen (p60, early T-cell activation antigen)
  • CD69 antigen
  • CD69 molecule
  • CD69
  • CLEC2CC-type lectin domain family 2 member C
  • C-type lectin domain family 2, member C
  • EA1
  • EA-1
  • early activation antigen CD69
  • early lymphocyte activation antigen
  • Early T-cell activation antigen p60
  • GP32/28
  • Leu23
  • Leukocyte surface antigen Leu-23
  • MLR-3
  • p60

Background

This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants. Transcript Variant: This variant (1) represents the longest transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CD69 Lysate (NBL1-08963) (0)

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Bioinformatics

Gene Symbol CD69
Entrez