CD69 Antibody (3190A) [Unconjugated]

CD69 was detected in immersion fixed paraffin-embedded sections of human Tonsil using Rabbit Anti-Human CD69, Monoclonal Antibody (Catalog # MAB11691) at 25ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

CD69 was detected in immersion fixed paraffin-embedded sections of human lymph node using Rabbit Anti-Human CD69 Monoclonal Antibody (Catalog # MAB11691) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CD69 was detected in immersion fixed paraffin-embedded sections of human spleen using Rabbit Anti-Human CD69 Monoclonal Antibody (Catalog # MAB11691) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western Blot shows lysates of human tonsil tissue, human thymus tissue and human CD4+ T cells. PVDF membrane was probed with 2 µg/ml of Rabbit Anti-Human CD69 Monoclonal Antibody (Catalog # MAB11691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CD69 at approximately 20-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IHC, mIF
• Clone: 3190A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CD69 Antibody (3190A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line, NS0-derived human CD69
  Gly64-Lys199
  Accession # Q07108
• Specificity: Detects recombinant human CD69 protein in Direct ELISA.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-10 ug/mL
  • Multiplex Immunofluorescence 25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD69 Antibody (3190A) [Unconjugated]

• activation inducer molecule (AIM/CD69)
• Activation inducer molecule
• AIM
• BL-AC/P26
• CD69 antigen (p60, early T-cell activation antigen)
• CD69 antigen
• CD69 molecule
• CD69
• CLEC2CC-type lectin domain family 2 member C
• C-type lectin domain family 2, member C
• EA1
• EA-1
• early activation antigen CD69
• early lymphocyte activation antigen
• Early T-cell activation antigen p60
• GP32/28
• Leu23
• Leukocyte surface antigen Leu-23
• MLR-3
• p60

Background

CD69, also known as Leu 23, AIM, EA-1, and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells, and platelets.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q07108()