CD68/SR-D1 Antibody (2449D) [Unconjugated]

CD68 was detected in immersion fixed paraffin-embedded sections of mouse Liver using Rabbit Anti-Mouse CD68, Monoclonal Antibody (Catalog #MAB101141) at 5ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm of Kupffer cells. Protocol available in COMET™ Panel Builder.​​

CD68 was detected in perfusion fixed-frozen sections of mouse Brain Medulla using Rabbit Anti-Mouse CD68, Monoclonal Antibody (Catalog#MAB101141) at 20ug/mL at 27° Celsius for 8minutes. Before incubation with the primary antibody, tissue underwent preprocessing by incubating tissue with Multi Staining Buffer (Lunaphore Catalog # BU06) for 5 minutes at room temperature followed by a 20-minute incubation in Tris-Buffered Saline + 0.2% Triton at room temperature. Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

CD68/SR-D1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Rabbit Anti-Mouse CD68/SR-D1 Monoclonal Antibody (Catalog # MAB101141) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Raw264.7 mouse cell line was stained with Rabbit Anti-Mouse CD68 Monoclonal Antibody (Catalog # MAB101141, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1x) (Catalog # FC004) and permeabilized with ice-cold methanol.

• Reactivity: Mu
• Applications: IHC, CyTOF-ready, Flow, mIF
• Clone: 2449D
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CD68/SR-D1 Antibody (2449D) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Chinese Hamster Ovary cell line, CHO-derived mouse CD68
  Asp21-Pro282
  Accession # P31996
• Specificity: Detects mouse CD68 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunohistochemistry 3-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Multiplex Immunofluorescence 5-20 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD68/SR-D1 Antibody (2449D) [Unconjugated]

• CD_antigen: CD68
• CD68 antigen
• CD68 molecule
• CD68
• DKFZp686M18236
• gp110
• LAMP4
• macrophage antigen CD68
• Macrosialin
• SCARD1
• scavenger receptor class D, member 1
• SRD1
• SR-D1

Background

Macrosialin (CD68) is a macrophage-restricted glycosylated transmembrane protein.  It belongs to the lamp family and carries out specialized functions in dedicated phagocytic cells.  Macrosialin binds to tissue- and organ-specific lectins to allow homing of macrophages to specific targets.  Its presence in macrophages is useful in diagnosing conditions related to proliferation or abnormality of these cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD68/SR-D1 Antibody (MAB101141)(2)

Publications using MAB101141

• Agueda-Oyarzabal, M;Isidor, MS;Pluci?ska, K;Ingerslev, LR;Dmytriyeva, O;Petersen, PSS;Laftih, S;Pontoppidan, AB;Henningsen, JB;Rupar, K;Brown, EL;Schwartz, TW;Barrès, R;Gerhart-Hines, Z;Schéele, CC;Emanuelli, B; Transcriptomic signatures of cold acclimated adipocytes reveal CXCL12 as a Brown autocrine and paracrine chemokine Molecular metabolism 2025-01-21 [PMID: 39848402] (In Situ Hybridization, Mouse)
• Ragnar O. Vilmundarson, Niloufar Heydarikhorneh, An Duong, Tiffany Ho, Kianoosh Keyhanian, Fariborz Soheili et al. Savior Siblings Might Rescue Fetal Lethality But Not Adult Lymphoma in Irf2bp2-Null Mice Frontiers in Immunology 2022-07-04 [PMID: 35865523] (Immunohistochemistry, Transgenic Mouse)

Bioinformatics

• Uniprot:
  • P31996()