CD63 Antibody (460305) [Unconjugated]

Images

 
CD63 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human CD63 Monoclonal Antibody (Catalog # MAB5048) at 25 µg/mL for 3 hours at room temperature. Cells were stained using ...read more
Human peripheral blood mononuclear cells were stained with Mouse Anti-Human CD63 Monoclonal Antibody (Catalog # MAB5048, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications CyTOF-ready, Flow, ICC/IF
Clone
460305
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

CD63 Antibody (460305) [Unconjugated] Summary

Immunogen
HEK293 human embryonic kidney cell line transfected with human CD63
Met1-Met238
Accession # P08962
Specificity
Stains human CD63 transfectants but not irrelevant transfectants.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD63
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 5-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Reviewed Applications
Read 1 Review rated 4
using
MAB5048 in the following applications:

Publications
Read Publication using
MAB5048 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD63 Antibody (460305) [Unconjugated]

  • CD63 antigen (melanoma 1 antigen)
  • CD63 antigen
  • CD63 molecule
  • CD63
  • Granulophysin
  • Lamp-3
  • Lysosomal-associated membrane protein 3
  • ME491
  • melanoma 1 antigen
  • Melanoma-associated antigen ME491
  • melanoma-associated antigen MLA1
  • MLA1lysosome-associated membrane glycoprotein 3
  • Ocular melanoma-associated antigen
  • OMA81H
  • tetraspanin-30
  • Tspan30
  • tspan-30
  • TSPAN30granulophysin

Background

CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of human CD63 share 67% and 65% amino acid sequence identity with mouse and rat CD63, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD63 Antibody (MAB5048)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(1)
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Filter By Species
Human
(1)
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Review for CD63 Antibody (MAB5048) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB5048:
Filter by Applications
Flow
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Flow Human 02/25/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 23072732
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23072732

Details

Detection NotesSee PMID 23072732

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23072732

FAQs for CD63 Antibody (MAB5048) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
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4
1
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1
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Anonymous
02/25/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CD63
Uniprot