CD5 Antibody (53-7.3) [Unconjugated]

• Reactivity: Mu
• Applications: Flow, IHC, IP, CyTOF-ready, Func
• Clone: 53-7.3
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CD5 Antibody (53-7.3) [Unconjugated] Summary

• Immunogen: Mouse thymus or spleen
• Specificity: Detects mouse CD5.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CD5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blocking B Cell Proliferation
  • Complement-dependent Cytotoxicity
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD5 Antibody (53-7.3) [Unconjugated]

• CD5 antigen (p56-62)
• CD5 antigen
• CD5 molecule
• CD5
• LEU1T-cell surface glycoprotein CD5
• Lymphocyte antigen T1/Leu-1
• T1

Background

Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a Group B member of the scavenger receptor cysteine-rich protein superfamily (1). CD5 is a 67 kDa type I transmembrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2-6). It is likely a ligand of the B cell differentiation antigen CD72 and is known to associate with CD6 (1). Mouse and human extracellular domains share 55% amino acid sequence indentity.

1. Martinez, V.G., et al. (2011) Pharmacol. Rev. 63:967.
2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
3. van Ewijk, W. et al. (1981) J. Immunol. 127:2594.
4. Hayakawa, K. et al. (1983) J. Exp. Med. 157:202.
5. Luo, W. et al. (1992) J. Immunol. 148:1630.
6. Lanier, L.L. et al. (1986) J. Immunol. 137:2735.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD5