CD5 Antibody (53-7.3) [DyLight 594]

• Reactivity: Mu
• Applications: Flow, IHC, IP, CyTOF-ready, Func
• Clone: 53-7.3
• Clonality: Monoclonal
• Host: Rat
• Conjugate: DyLight 594

CD5 Antibody (53-7.3) [DyLight 594] Summary

• Immunogen: Mouse thymus or spleen
• Specificity: Detects mouse CD5.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CD5
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Blocking B Cell Proliferation
  • Complement-dependent Cytotoxicity
  • CyTOF-reported
  • Flow Cytometry
  • Immunohistochemistry
  • Immunoprecipitation
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

0

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CD5 Antibody (53-7.3) [DyLight 594]

• CD5 antigen (p56-62)
• CD5 antigen
• CD5 molecule
• CD5
• LEU1T-cell surface glycoprotein CD5
• Lymphocyte antigen T1/Leu-1
• T1

Background

In humans, CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD5