CD47 Antibody (miap301) - BSA Free

Flow Cytometry: CD47 Antibody (miap301) [NBP1-43407] - Staining of BALB/c splenocytes.

• Reactivity: Mu
• Applications: WB, Flow, IHC, IP
• Clone: miap301
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

CD47 Antibody (miap301) - BSA Free Summary

• Description: Novus Biologicals Rat CD47 Antibody (miap301) - BSA Free (NBP1-43407) is a monoclonal antibody validated for use in IHC, WB, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody was CD47.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: CD47
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 ug/10^6 cells
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
• Application Notes: This miap30 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Aqueous , may contain carrier protein/stabilizer.
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CD47 Antibody (miap301) - BSA Free

• antigen identified by monoclonal 1D8
• Antigenic surface determinant protein OA3
• CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
• CD47 antigen
• CD47 glycoprotein
• CD47 molecule
• CD47
• IAP
• IAPintegrin associated protein
• Integrin-associated protein
• leukocyte surface antigen CD47
• MER6
• MER6integrin-associated signal transducer
• OA3
• Protein MER6
• Rh-related antigen

Background

The monoclonal antibody miap301 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with 3 integrins CD51/CD61, CD41/CD61. It is also known to be the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells. No crossreactivity is seen against human CD47.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD47
• Entrez:
  • 961(Human)
• Uniprot:
  • Q08722()