CD47 Antibody (IAP/964)

Immunocytochemistry/Immunofluorescence: CD47 Antibody (IAP/964) [NBP2-44408] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells. CD47 Mouse Monoclonal Antibody (IAP/964) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

Flow Cytometry: CD47 Antibody (IAP/964) [NBP2-44408] - Flow Cytometric Analysis of live-Jurkat cells. CD47 Mouse Monoclonal Antibody (IAP/964) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Immunocytochemistry/Immunofluorescence: CD47 Antibody (IAP/964) [NBP2-44408] - Immunofluorescence Analysis of Live Jurkat cells. CD47 Mouse Monoclonal Antibody (IAP/964) followed by goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

• Reactivity: Hu, Mu
• Applications: ELISA, Flow, ICC/IF
• Clone: IAP/964
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD47 Antibody (IAP/964) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08974)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human CD47 protein (Uniprot: Q08722)
• Localization: Cell surface, cytoplasmic
• Specificity: This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD47
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
  ELISA: For coating, order antibody without BSA
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD47 Antibody (IAP/964)

• antigen identified by monoclonal 1D8
• Antigenic surface determinant protein OA3
• CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
• CD47 antigen
• CD47 glycoprotein
• CD47 molecule
• CD47
• IAP
• IAPintegrin associated protein
• Integrin-associated protein
• leukocyte surface antigen CD47
• MER6
• MER6integrin-associated signal transducer
• OA3
• Protein MER6
• Rh-related antigen

Background

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD47
• Uniprot:
  • Q08722()
  • Q08722()