CD45RB Antibody (1067419) [Unconjugated]

CD45RB was detected in immersion fixed paraffin-embedded sections of Human Lymph Node using Mouse Anti-Human CD45RB Monoclonal Antibody (Catalog # MAB10477) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 1067419
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CD45RB Antibody (1067419) [Unconjugated] Summary

• Immunogen: Chinese Hamster Ovary cell line, CHO-derived human CD45RB
  Gln26-Lys463
  Accession # P08575
• Specificity: Detects human CD45RB in direct ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD45RB Antibody (1067419) [Unconjugated]

• B220
• CD45 antigen
• CD45R
• CD45RB
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor-like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1-3). Multiple splicing isoforms of exon 4 (A), 5 (B), and 6 (C) are expressed according to cell type, developmental stage and antigenic exposure (1-5). The longest form, CD45RABC (called B220 in mouse) is expressed on B lymphocytes and the shortest form, CD45R0 is expressed on memory cells (5). Isoform CD45RB contains exon 5 (B exon) in the extracellular domain, which shares 41% and 40% homology with the ECD of mouse and rat CD45RB. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6-8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Interaction of galaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates of CD45 (4, 9). Within the immunological synapse, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8-10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11-14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

1. Anderson, J.N. et al. (2004) FASEB J. 18:8.
2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
4. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
5. Ralph, S.J. et al. (1987) EMBO J. 6:1251.
6. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
7. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
8. McNiell, L. et al. (2007) Immunity 27:425.
9. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
10. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
11. Zhu, J.W. et al. (2008) Immunity 28:183.
12. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
13. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
14. Cross, J.L. et al. (2008) J. Immunol. 180:8020.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P08575()