CD45RA Antibody (PG167A)

Flow Cytometry: CD45RA Antibody (PG167A) [NBP2-60912] - Obtained with pig leukocytes

Product Discontinued

CD45RA Antibody (PG167A) Summary

• Immunogen: Pig mononuclear cells
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRC
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.
• Purity: Unpurified

Notes

This monoclonal antibody is made in ascites form using mice. The ascites is collected and centrifuged to remove cells and large particles. The ascitic fluid is purified further by filtration through sequential filters with the last filter at 2 um. The concentration of the monoclonal antibody present in the ascites is determined with commercial radial immunodiffusion kits (1mg/ml).

Alternate Names for CD45RA Antibody (PG167A)

• B220
• CD45 antigen
• CD45R
• CD45RA
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

The CD45 molecule is comprised of different glycoproteins ranging from 180-240 kD. Expression of CD45 is found on all hemopoietic cells. Detection of the different isoforms can distinguish between different cell forms i.e. naive T cells and memory T cells. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. The functional activity of each CD45 isotype also varies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRC