CD45RA Antibody (OX-33) [PE] - (B Cells only)

Product Image: CD45RA Antibody (OX-33) [PE] [NB100-65000PE] - Vial of PE conjugated antibody. PE has two excitation maxima, 498 nm excited by the Blue laser (488 nm) and 565 nm excited by the Yellow-Green laser (561 nm). Both result in emission at 578 nm.

• Reactivity: Rt
• Applications: Flow, IHC
• Clone: OX-33
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

CD45RA Antibody (OX-33) [PE] - (B Cells only) Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Purified Rat spleen L-CA
• Localization: Membrane; single pass type I membrane protein.
• Specificity: NB100-65000 is directed against a high molecular weight band of the leucocyte common antigen (LCA). The MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labelling in bone marrow and none on thymocytes (2).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRC
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for CD45RA Antibody (OX-33) [PE] - (B Cells only)

• B220
• CD45 antigen
• CD45R
• CD45RA
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

The CD45 molecule is comprised of different glycoproteins ranging from 180-240 kD. Expression of CD45 is found on all hemopoietic cells. Detection of the different isoforms can distinguish between different cell forms i.e. naive T cells and memory T cells. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. The functional activity of each CD45 isotype also varies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRC